| 引用本文: | 孙志,周霖,左莉华,张俊,王肖辉,康建,聂会娟,李卓伦,贾清泉,刘丽伟,师莹莹,张晓坚.基于UHPLC-Q-Orbitrap HRMS技术的益心颗粒化学成分研究[J].中国现代应用药学,2019,36(10):1197-1204. |
| SUN Zhi,ZHOU Lin,ZUO Lihua,ZHANG Jun,WANG Xiaohui,KANG Jian,NIE Huijuan,LI Zhuolun,JIA Qingquan,LIU Liwei,SHI Yingying,ZHANG Xiaojian.Chemical Constituents Study of Yixin Granules Based on UHPLC-Q-Orbitrap HRMS Technology[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(10):1197-1204. |
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| 基于UHPLC-Q-Orbitrap HRMS技术的益心颗粒化学成分研究 |
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孙志1,2, 周霖1,2, 左莉华1,2, 张俊1,2, 王肖辉3, 康建1,2, 聂会娟1,2, 李卓伦1,2, 贾清泉1,2, 刘丽伟1,2, 师莹莹1,2, 张晓坚1,2
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1.郑州大学第一附属医院, 药学部, 郑州 450052;2.河南省精准临床药学重点实验室, 郑州 450052;3.郑州大学第一附属医院, 超声科, 郑州 450052
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| 摘要: |
| 目的 建立一种快速、准确识别益心颗粒中复杂化学成分的定性分析方法。方法 主要通过UHPLC-Q-Orbitrap HRMS捕捉化合物的精准分子量以及多级碎片离子信息，同时将其与对照品的保留时间和质谱信息进行比对，并结合相关参考文献或Chemical Book等数据库信息最终实现对未知化合物的快速定性。结果 从益心颗粒中共鉴定出43种化学成分，主要包括有机酸类、内酯类、木脂素类、甾体皂苷类和其他类。结论 该方法可快速、准确、系统地识别益心颗粒中多种化学成分，并为其质量控制、药效物质基础研究及进一步的临床应用提供扎实的理论依据和科学的研究思路。 |
| 关键词: 益心颗粒 UHPLC-Q-Orbitrap HRMS 化学成分 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.10.006 |
| 分类号:R284.1 |
| 基金项目:河南省高等学校重点科研项目（18A360022，19A320070）；常州四药临床药学科研基金（CZSYJJ16030） |
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| Chemical Constituents Study of Yixin Granules Based on UHPLC-Q-Orbitrap HRMS Technology |
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SUN Zhi1,2, ZHOU Lin1,2, ZUO Lihua1,2, ZHANG Jun1,2, WANG Xiaohui3, KANG Jian1,2, NIE Huijuan1,2, LI Zhuolun1,2, JIA Qingquan1,2, LIU Liwei1,2, SHI Yingying1,2, ZHANG Xiaojian1,2
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1.The First Affiliated Hospital of Zhengzhou University, Department of Pharmacy, Zhengzhou 450052, China;2.Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou 450052, China;3.The First Affiliated Hospital of Zhengzhou University, Department of Ultrasonography, Zhengzhou 450052, China
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| Abstract: |
| OBJECTIVE To establish a rapid and accurate qualitative analysis method for the identification of complex chemical constituents in Yixin granules.METHODS UHPLC-Q-Orbitrap HRMS was used to capture the precise molecular weight and multi-level fragment ion information of the compounds. At the same time, the retention time and mass spectrometry information was compared with the reference substance, while combining the relevant references or the database information such as Chemical Book and so on. Therefore, a rapid and accurate determination of the unknown compounds was achieved. RESULTS Forty-three chemical constituents were identified from Yixin granules, including organic acids, lactones, lignans, steroidal saponins and others. CONCLUSION The established method is rapid, accurate and systematic for the identification of various chemical constituents in Yixin granules, which provides a solid theoretical basis and scientific research ideas for its quality control, pharmacodynamic substance basis research and further clinical application. |
| Key words: Yixin granules UHPLC-Q-Orbitrap HRMS chemical constituents |
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