N-乙酰基转移酶活性测定方法综述

王晓华; 寇佳昕; 宋佩佩; 刘亚琦; 袁圆; 王璨; 秦红岩<sup>*</sup>

引用本文:	王晓华,寇佳昕,宋佩佩,刘亚琦,袁圆,王璨,秦红岩.N-乙酰基转移酶活性测定方法综述[J].中国现代应用药学,2019,36(2):240-244.
	WANG Xiaohua,KOU Jiaxin,SONG Peipei,LIU Yaqi,YUAN Yuan,WANG Can,Qin Hongyan.Methodological Review about the Activity Determination Assay of N-Acetyltransferase[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(2):240-244.

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N-乙酰基转移酶活性测定方法综述
王晓华¹, 寇佳昕², 宋佩佩², 刘亚琦³, 袁圆³, 王璨², 秦红岩¹
1.兰州大学第一医院药剂科, 兰州 730000;2.兰州大学, 第二临床医学院, 兰州 730000;3.兰州大学, 第一临床医学院, 兰州 730000

摘要:

N-乙酰基转移酶（N-acetyltransferase，NAT）是人体最为重要的Ⅱ相药物代谢酶，在体内药物代谢和毒物解毒方面具有重要作用。NAT的活性存在明显的种族和个体差异，其活性强弱可直接影响药物疗效和毒性反应。NAT活性测定可用于评估人体经乙酰化代谢药物的体内处置和代谢程度。通过查阅文献和相关资料，本文系统归纳了NAT的活性测定方法并对特点和优势进行了总结，可为NAT活性评估提供方法学参考，为基于NAT活性的临床个体化用药提供依据。

关键词: N-乙酰基转移酶活性评估药物代谢

DOI：10.13748/j.cnki.issn1007-7693.2019.02.024

分类号:R927.2

基金项目:甘肃省自然科学基金项目（1606RJZA117）；甘肃省中医药管理局项目（GZK-2018-46）；甘肃省卫生行业科研计划管理基金项目（GWGL2013-25）

Methodological Review about the Activity Determination Assay of N-Acetyltransferase

WANG Xiaohua¹, KOU Jiaxin², SONG Peipei², LIU Yaqi³, YUAN Yuan³, WANG Can², Qin Hongyan¹

1.Department of Pharmacy, First Hospital of Lanzhou University, Lanzhou 730000, China;2.Lanzhou University, Second Clinical Medical College, Lanzhou 730000, China;3.Lanzhou University, First Clinical Medical College, Lanzhou 730000, China

Abstract:

N-acetyltransferase (NAT) is an important enzyme of phase Ⅱ drug metabolism of the body, it also plays a vital role in drug metabolism and noxious substances detoxification. NAT's activity differs markedly among individuals and races, which may be responsible for the different herapeutic effect and toxic reaction of certain drugs. NAT's activity can be used to evaluate the ability of acetyl metabolism and drug disposition. This review systematically summarized the methods for NAT activity assay by consulting literatures and the related information, the features as well as strengths of the described methods for NAT activity assay are also summarized here. This present review may provides methodological evidence for NAT activity evaluation, it may also provide a novel basis for personalized drug therapy in clinical.

Key words: N-acetyltransferase activity assay drug metabolism