| 引用本文: | 车景超,田英,徐平,王鹏光.银桂抗炎合剂质量标准研究[J].中国现代应用药学,2019,36(8):961-964. |
| CHE Jingchao,TIAN Ying,XU Ping,WANG Pengguang.Study on Quality Standard for Yingui Kangyan Mixture[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(8):961-964. |
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| 摘要: |
| 目的 建立银桂抗炎合剂的质量标准。方法 采用薄层色谱法对制剂中的丹参、紫花地丁进行定性鉴别，采用HPLC对主要有效成分盐酸小檗碱进行含量测定。结果 丹参、紫花地丁的薄层色谱法鉴别专属性强，阴性无干扰；含量测定结果显示，盐酸小檗碱浓度在7.80~78.00 μg·mL-1（r=0.999 3）内与峰面积呈良好的线性关系，平均加样回收率为98.09%（n=6），RSD=1.09%。结论 本方法简便、专属性强，重复性好，可用于银桂抗炎合剂的质量控制。 |
| 关键词: 银桂抗炎合剂 质量标准 薄层色谱法 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.08.013 |
| 分类号:R284.1;R917.101 |
| 基金项目: |
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| Study on Quality Standard for Yingui Kangyan Mixture |
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CHE Jingchao, TIAN Ying, XU Ping, WANG Pengguang
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Shandong Qingdao Hospital of Integrated Traditional and Western Medicine, Qingdao 266002, China
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| Abstract: |
| OBJECTIVE To establish the quality standard of Yingui Kangyan mixture. METHODS Salvia miltiorrhiza and Viola yedoensis Makino in Yingui Kangyan mixture were identified with the method of TLC. The concentration of berberine hydrochloride was determined with the method of HPLC. RESULTS TLC identifications of Salvia miltiorrhiza and Viola yedoensis Makino were specific, and no interference was observed in negative control samples. The calibration curve of berberine hydrochloride demonstrated a good linear relationship in the range of 7.80-78.00 μg·mL-1 (r=0.999 3), and the average recovery rate was 98.09%(n=6), RSD=1.09%. CONCLUSION The method is simple, specific, reproducible, and suitable for quality control of Yingui Kangyan mixture. |
| Key words: Yingui Kangyan mixture quality standard TLC HPLC |
