| 引用本文: | 马霞,丁军霞,袁菊丽,邓淑芳,刘洁丽,刘东玲,王亚丽.当归茎叶与根对小鼠溶血性贫血模型的影响[J].中国现代应用药学,2019,36(3):297-302. |
| MA Xia,DIND Junxia,YUAN Juli,DENG Shufang,LIU Jieli,LIU Dongling,WANG Yali.Effects of Angelica Sinensis Stems and Leaves and Angelica Sinensis Roots on Hemolytic Anemia Model Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(3):297-302. |
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| 当归茎叶与根对小鼠溶血性贫血模型的影响 |
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马霞1,2, 丁军霞3, 袁菊丽1, 邓淑芳1,4, 刘洁丽1,4, 刘东玲1,4,5, 王亚丽1,4,5
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1.甘肃中医药大学, 兰州 730000;2.仪陇县中医医院, 四川 南充 637600;3.新疆生产建设兵团第二师库尔勒医院, 新疆 库尔勒 841000;4.甘肃省当归产业发展研究院, 兰州 730000;5.甘肃省中药质量与标准研究重点实验室培育基地, 兰州 730000
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| 摘要: |
| 目的 探讨当归茎叶与根对溶血性贫血小鼠的干预作用。方法 将90只小鼠随机分为9组：空白组，模型组，阳性组，当归茎叶水煎液组（高、中、低剂量），当归根水煎液组（高、中、低剂量），每组10只，♀♂各半。采用乙酰苯肼（acetyl phenyl hydrazine，APH）制造溶血性贫血模型（1，4，7 d皮下注射含有APH的生理盐水，APH剂量为0.2，0.1，0.1 g·kg-1），造模第1天给予当归根水煎液与茎叶水煎液灌胃治疗（0.02 mL·g-1），13 d后取样，检测其血常规（Hb、RBC、PLT）、脏器指数（脾、胸腺指数）、生化指标（G6PD、GR、IL-2和EPO）以及观察肝、脾病理切片（苏木精-伊红染色）。结果 与模型组比较，当归茎叶水煎液与当归根水煎液均能显著升高Hb、RBC、PLT，降低脾指数，提高胸腺指数，升高小鼠血液中G6PD、GR、IL-2、EPO的含量（P<0.05或<0.01）。另外，病理切片结果显示，当归茎叶水煎液和当归根水煎液均可减轻APH所致小鼠肝脏、脾脏细胞的点状坏死及瘀血。结论 当归茎叶水煎液与当归根水煎液对溶血性贫血小鼠均具有一定的改善作用。 |
| 关键词: 当归茎叶 当归根 血虚 资源利用 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.03.008 |
| 分类号:R285.5 |
| 基金项目:甘肃省科技支撑计划项目（144FKCA078）；甘肃省发改委当归种植、加工炮制过程质量控制相关性关键核心技术的研究与开发 |
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| Effects of Angelica Sinensis Stems and Leaves and Angelica Sinensis Roots on Hemolytic Anemia Model Mice |
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MA Xia1,2, DIND Junxia3, YUAN Juli1, DENG Shufang1,4, LIU Jieli1,4, LIU Dongling1,4,5, WANG Yali1,4,5
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1.Gansu University of TCM, Lanzhou 730000, China;2.Yilong County Hospital of Traditional Chinese Medicine, Nanchong 637600, China;3.Korla Hospital, Second Division of Xinjiang Production and Construction Corps, Korla 841000, China;4.Angelica Industry in Gansu Province Development Institute, Lanzhou 730000, China;5.Key Laboratory of Traditional Chinese Medicine Quality and Standard of Gansu Province, Lanzhou 730000, China
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| Abstract: |
| OBJECTIVE To study the effect of Angelica sinensis stems and leaves and Angelica sinensis roots on hemolytic anemia model mice. METHODS Ninety mice were randomly divided into 9 groups:control group, model group, positive group, and Angelica sinensis stems and leaves(high, middle and low dose) and Angelica sinensis roots(high, middle and low dose) groups, 10 mice in each group, half male and half female. The animal model of hemolytic anemia was made by using acetyl phenyl hydrazine(APH)(1, 4, 7 d hypodermic inject saline containing 0.2, 0.1, 0.1 g·kg-1 APH, respectively). On the first day of modeling, Angelica sinensis roots decoction, Angelica sinensis stems and leaves decoction were given to mice by gavage(0.02 mL·g-1). Samples were taken 13 d later to test their blood routine(Hb, RBC, PLT), organ index(spleen index, thymus index), biochemical indicators(G6PD, GR, IL-2 and EPO), and to observe pathological sections of liver and spleen with HE staining. RESULTS Compared with the model group, Angelica sinensis stems and leaves, roots decoction could significantly increase Hb, RBC, PLT, decrease spleen index and improve the thymus index. Besides, increased the levels of G6PD, GR, IL-2 and EPO in the blood of mice(P<0.05 or <0.01). In addition, the results of pathological sections showed that Angelica sinensis stems and leaves decoction, Angelica sinensis roots decoction both reduced the point necrosis and congestion of liver and spleen cells in mouse induced by APH. CONCLUSION The Angelica sinensis stems and leaves, and Angelica sinensis roots have improving effect on hemolytic anemia mice. |
| Key words: Angelica sinensis stems and leaves Angelica sinensis roots blood deficiency resource utilization |
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