| 引用本文: | 鲁一航,陈礼迎,李一凡,张一帆,王高荣,何洁.卟啉修饰的壳聚糖光敏新材料的制备与表征[J].中国现代应用药学,2019,36(1):20-25. |
| LU Yihang,CHEN Liying,LI Yifan,ZHANG Yifan,WANG Gaorong,HE Jie.Synthesis and Characterization of the Chitosan-Porphyrin Complexes[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(1):20-25. |
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| 摘要: |
| 目的 合成卟啉修饰的壳聚糖光敏新材料，对其进行表征，并优化其合成工艺。方法 以溴己烷苯基卟啉衍生物和壳聚糖为原料，以K2CO3为碱，采用四丁基溴化铵为相转移催化剂，合成卟啉-壳聚糖复合物，采用茚三酮法测定卟啉接枝率。通过单因素实验研究反应温度、反应时间、混合溶剂比例、催化剂当量等对接枝率的影响，采用正交实验法优化获得最佳制备工艺条件。结果 最佳合成条件为温度55℃、反应时间为5.5 h、混合溶剂中水和氯仿的比例为1：2、催化剂为1当量、无机碱使用K2CO3，卟啉的平均接枝率为41.30%。结论 通过共价偶联合成卟啉修饰壳聚糖光敏新材料的方法可行。 |
| 关键词: 卟啉 壳聚糖 表征 正交实验 接枝率 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.01.005 |
| 分类号:R914.5 |
| 基金项目:2017年浙江省中医药科技计划项目（2017ZB027）；2017年浙江省大学生科技创新活动计划（新苗人才计划）（2017R410010）；2016年浙江省大学生科技创新活动计划（新苗人才计划）（2016R410018）；2011年浙江中医药大学校级科研基金（2011ZZ12） |
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| Synthesis and Characterization of the Chitosan-Porphyrin Complexes |
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LU Yihang, CHEN Liying, LI Yifan, ZHANG Yifan, WANG Gaorong, HE Jie
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College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China
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| Abstract: |
| OBJECTIVE To synthesis and characterize the chitosan-porphyrin complexes(CS-BHP), and to optimize the experimental condition. METHODS CS-BHP was synthesized from 5-p-(6-bromohexylaminophenyl)-10,15,20-triphenyl porphyrin(BHP) and chitosan by using K2CO3 as a base and tetrabutylammonium bromide as a phase transfer catalyst. The porphyrin grafting rate of CS-BHP was detected with ninhydrin by UV-Vis. In the single factor analysis, the effect of temperature, reaction time, the volume ratio of water to chloroform in the component solvent, equivalent of the catalyst were investigated. The orthogonal test was used to optimize the condition of synthesis. RESULTS The optimized condition of the reaction was as follows:the temperature was 55℃, the reaction time was 5.5 h, the volume ratio of water to chloroform in the component solvent was 1:2, catalyst was 1 equivalent, and K2CO3 was used as base, the average porphyrin grafting rate of CS-BHP was 41.30%. CONCLUSION It is feasible to synthesize CS-BHP by the method of covalent coupling. |
| Key words: porphyrin chitosan characterization orthogonal test grafting rate |
