| 引用本文: | 王君燕,童晔玲,赵文慧.獐牙菜苦苷的抗炎活性及其对NF-κB通路相关因子p65和IKK-α表达的影响[J].中国现代应用药学,2018,35(12):1817-1820. |
| WANG Junyan,TONG Yeling,ZHAO Wenhui.Anti-inflammatory Activity of Swertiamarin and Its Effect on the Expression of NF-κB Pathway-related Factors p65 and IKK-α[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(12):1817-1820. |
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| 摘要: |
| 目的 观察獐牙菜苦苷（Swertiamarin）的抗炎活性以及对核转录因子-κB（NF-κB）通路的调控作用。方法 采用脂多糖（LPS）诱导小鼠巨噬细胞RAW264.7构建体外炎症模型，CCK-8法观察獐牙菜苦苷对细胞活性的影响，ELISA法检测其对炎症相关因子肿瘤坏死因子-α（TNF-α）、白介素-6（IL-6）表达的影响，RT-PCR法测定其对NF-κB通路相关因子p65和IKK-α的mRNA表达的影响。结果 100 μg·mL-1獐牙菜苦苷能降低细胞存活率，3.125~50 μg·mL-1浓度对细胞存活率无影响；25~50 μg·mL-1獐牙菜苦苷能降低炎症相关因子TNF-α及IL-6的释放；12.5~50 μg·mL-1獐牙菜苦苷能抑制p65的mRNA表达，50 μg·mL-1獐牙菜苦苷能抑制IKK-α的mRNA表达。结论 獐牙菜苦苷对LPS诱导的炎症模型中TNF-α、IL-6的生成具有抑制作用，其机制可能与抑制NF-κB通路相关因子p65、IKK-α的表达有关。 |
| 关键词: 獐牙菜苦苷 巨噬细胞 抗炎活性 核转录因子-κB |
| DOI：10.13748/j.cnki.issn1007-7693.2018.12.013 |
| 分类号:R285.5 |
| 基金项目:浙江省医药卫生科技计划项目（2016KYA003） |
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| Anti-inflammatory Activity of Swertiamarin and Its Effect on the Expression of NF-κB Pathway-related Factors p65 and IKK-α |
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WANG Junyan1, TONG Yeling2, ZHAO Wenhui2
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1.Zhejiang Hospital, Hangzhou 310030, China;2.Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China
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| Abstract: |
| OBJECTIVE To observe the anti-inflammatory activity of Swertiamarin and its regulation effect on the nuclear transcription factor-κB(NF-κB) pathway. METHODS Lipopolysaccharid(LPS) induced murine macrophage raw 264.7 cell was used to establish an inflammatory model in vitro. CCK-8 method was used to observe the effect of Swertiamarin on cell activity. ELISA was used to detect its effect on the expression of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6). RT-PCR was used to detect its effect on mRNA expression of NF-κB pathway related factors p65 and Ikk-α. RESULTS The 100 μg·mL-1 Swertiamarin could decrease the cell survival rate, and 3.125-50 μg·mL-1 had no effect on the cell survival rate. The 25-50 μg·mL-1 Swertiamarin could reduce the release of TNF-α and IL-6. It was found that 12.5-50 μg·mL-1 Swertiamarin could inhibit the mRNA expression of p65, and the mRNA expression of Ikk-α could be inhibited by 50 μg·mL-1 Swertiamarin. CONCLUSION Swertiamarin has inhibitory effect on TNF-α and IL-6 production in LPS-induced inflammatory model, and its mechanism may be related to the inhibition of the expression of p65 and IKK-α, which are related factors of NF-κB pathway. |
| Key words: Swertiamarin macrophage anti-inflammatory activity NF-κB |
