| 引用本文: | 金朦娜,彭彦,王秀秀,汪霞,杨直.UPLC同时测定中成药和保健食品中16种非法添加的抗过敏类药物[J].中国现代应用药学,2019,36(2):186-190. |
| JIN Mengna,PENG Yan,WANG Xiuxiu,WANG Xia,YANG Zhi.Determination of 16 Anti-allergic Drugs in Chinese Traditional Patent Medicine and Health Food by UPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(2):186-190. |
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| 摘要: |
| 目的 建立UPLC测定中成药和保健食品中16种非法添加的抗过敏类药物。方法 采用Waters BEH C18色谱柱（100 mm×2.1 mm，1.7 μm），以甲醇-20 mmol·L-1磷酸二氢钠（用磷酸调节pH值至3.0）为流动相，梯度洗脱，检测波长为220 nm。结果 16种抗过敏类药物在2.5~40 μg·mL-1内呈良好线性关系（r2>0.999），平均回收率为90.77%~113.35%，RSD为0.06%~1.21%；定量限为0.18~3.92 μg·mL-1，检出限为0.09~0.78 μg·mL-1。结论 该方法简便、灵敏度高，可有效地判断中成药及保健品中是否非法掺入16种抗过敏类药物。 |
| 关键词: 超高效液相色谱 抗过敏药物 非法添加 中成药 保健品 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.02.012 |
| 分类号:R917.101 |
| 基金项目:浙江省食品药品监管系统科技计划项目（BH201601） |
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| Determination of 16 Anti-allergic Drugs in Chinese Traditional Patent Medicine and Health Food by UPLC |
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JIN Mengna, PENG Yan, WANG Xiuxiu, WANG Xia, YANG Zhi
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Hangzhou Institute for Food and Drug Control, Hangzhou 310022, China
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| Abstract: |
| OBJECTIVE To develop a UPLC method to determine 16 anti-allergic drugs illegally added in chinese traditional patent medicine and health food. METHODS The separation was performed on a Waters BEH C18 column (100 mm×2.1 mm, 1.7 μm) with the mobile phase consisted of methanol-20 mmol·L-1 sodium dihydrogen phosphate solution (adjusted to pH 3.0 with phosphoric acid) in a gradient elution mode, the detector wavelength was 220 nm. RESULTS The results of 16 anti-allergic drugs showed good linearity in the range of 2.5-40 μg·mL-1(r2>0.999). The average recoveries were from 90.77% to 113.35%, with the RSD from 0.06% to 1.21%. The limits of quantifications were among 0.18-3.92 μg·mL-1, and the limits of detections were among 0.09-0.78 μg·mL-1. CONCLUSION This method is simple and sensitive, and it can effectively judge whether the chinese patent medicines and health products are illegally mixed with these 16 anti-allergic drugs. |
| Key words: UPLC anti-allergic drugs illegal addition chinese traditional patent medicine health foods |
