| 引用本文: | 孙志,赵灵灵,左莉华,康建,郭三星,张素枝,周霖,吕鹏,张晓坚.UHPLC-Q-Orbitrap HRMS鉴定丹灯通脑软胶囊中多种化学成分[J].中国现代应用药学,2019,36(2):191-199. |
| SUN Zhi,ZHAO Lingling,ZUO Lihua,KANG Jian,GUO Sanxing,ZHANG Suzhi,ZHOU Lin,LYU Peng,ZHANG Xiaojian.Identification of Various Chemical Constituents in Dandeng Tongnao Capsule by UHPLC-Q-Orbitrap HRMS[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(2):191-199. |
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| 摘要: |
| 目的 应用超高效液相色谱-四级杆/静电场轨道阱高分辨质谱（UHPLC-Q-Orbitrap HRMS）对丹灯通脑胶囊中的化学成分进行快速分析鉴别。方法 采用ACQUITY UPLC® BEH C18色谱柱（2.1 mm×50 mm，1.7 μm），乙腈-0.1%甲酸水为流动相进行梯度洗脱，分离各个化学成分，质谱采用全扫描模式，正负离子同时扫描检测色谱流出物。根据质谱提供的化合物精准分子量、多级碎片离子信息，结合相关参考文献和数据库信息，同时与对照品的相对保留时间和质谱数据进行比对，定性鉴定化合物。结果 共鉴定出59种化学成分，主要包括黄酮类、醌类、有机酸类等化学成分，并对其药材来源进行了归属。结论 利用UHPLC-Q-Orbitrap HRMS建立的鉴别方法，能够准确、系统、快速地鉴定丹灯通脑胶囊中的多种化学成分，为研究丹灯通脑胶囊药效物质基础及其质量标准提供依据和参考。 |
| 关键词: 丹灯通脑软胶囊 超高效液相色谱-四级杆/静电场轨道阱高分辨质谱 化学成分分析 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.02.013 |
| 分类号:R284.1;R917.101 |
| 基金项目: |
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| Identification of Various Chemical Constituents in Dandeng Tongnao Capsule by UHPLC-Q-Orbitrap HRMS |
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SUN Zhi, ZHAO Lingling, ZUO Lihua, KANG Jian, GUO Sanxing, ZHANG Suzhi, ZHOU Lin, LYU Peng, ZHANG Xiaojian
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The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China
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| Abstract: |
| OBJECTIVE To identify the chemical constituents in Dandeng Tongnao capsule by ultra high performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrometry(UHPLC-Q-Orbitrap HRMS). METHODS The ACQUITY UPLC® BEH C18 chromatographic column(2.1 mm×50 mm, 1.7 μm) was used for UPLC, and the acetonitrile-0.1% formic acid water was used as mobile phase to separate various chemical constituents by gradient elution separation. Chromatographic effluent was detected by UHPLC-Q-Orbitrap HRMS full scan in positive and negative ion mode. According to precise molecular weight of compounds and multistage fragment ion information detected by UHPLC-Q-Orbitrap HRMS, reference and database information, it could be analyzed accurate characterization of the compound compared with relative retention time and mass spectrometry data of control sample. RESULTS Fifty-nine chemical constituents which mainly including flavonoids, quinones, and organic acids were identified and classified. CONCLUSION A variety of chemical constituents can be accurately identified by UHPLC-Q-Orbitrap HRMS. The results can be consulted by the further study of the pharmacodynamic basis and quality standard of Dandeng Tongnao capsule. |
| Key words: dandeng tongnao capsule UHPLC-Q-Orbitrap HRMS chemical constituents analysis |
