| 引用本文: | 郑旭霞,刘培建.纤维素键合手性固定相拆分盐酸奈福泮对映体[J].中国现代应用药学,2019,36(3):278-280. |
| ZHENG Xuxia,LIU Peijian.Separation of Nefopam Hydrochloride with a Bonded Cellulose Chiral Stationary Phase[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(3):278-280. |
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| 摘要: |
| 目的 建立一种用键合纤维素手性固定相的HPLC拆分盐酸奈福泮对映异构体。方法 盐酸奈福泮的分离使用Chiralpak IC色谱柱（250 mm×4.6 mm，5 μm），流动相为正己烷-异丙醇（90：10，0.1% DEA），流速为0.8 mL·min-1，检测波长267 nm，柱温25℃。结果 盐酸奈福泮对映体在键合纤维素手性固定相上能够完全分离，分离度为6.53。结论 该方法可快速实现盐酸奈福泮对映体的分离。 |
| 关键词: 高效液相色谱法 Chialpak IC手性柱 对映体分离 盐酸奈福泮 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.03.004 |
| 分类号:R917.101 |
| 基金项目: |
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| Separation of Nefopam Hydrochloride with a Bonded Cellulose Chiral Stationary Phase |
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ZHENG Xuxia, LIU Peijian
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Luoyang Orthopedic Hospital of Henan Province, Zhengzhou 450000, China
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| Abstract: |
| OBJECTIVE To develop an HPLC method with a bonded cellulose chiral stationary phase for the chiral separating enantiomers of nefopam hydrochloride. METHODS Nefopam hydrochloride was separated by a Chiralpak IC (250 mm×4.6 mm, 5 μm) at 25℃ and detected at 267 nm, the mobile phase was n-hexane-isopropanol(90:10, 0.1% DEA) at a flow rate of 0.8 mL·min-1. RESULTS Nefopam hydrochloride enantiomers were separated on a cellulose bonded chiral stationary phase and the resolution was 6.53. CONCLUSION The method can be used for quickly separating the enantiomers of nefopam hydrochloride. |
| Key words: HPLC Chialpak IC chiral column enantiomeric separation nefopam hydrochloride |
