| 引用本文: | 沈伟锋,朱智慧,朱瑞,赵华军.Jatamanvaltrate P对乳腺癌细胞T47D增殖的影响及机制研究[J].中国现代应用药学,2019,36(1):10-14. |
| SHEN Weifeng,ZHU Zhihui,ZHU Rui,ZHAO Huajun.Effect and Mechanism of Jatamanvaltrate P on Proliferation of T47D Cells to Breast Cancer[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(1):10-14. |
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| 摘要: |
| 目的 考察蜘蛛香中环烯醚萜类化合物Jatamanvaltrate P对乳腺癌细胞T47D的增殖影响及作用机制。方法 MTT法检测Jatamanvaltrate P对乳腺癌细胞T47D和BT-20增殖的影响；DAPI染色和流式细胞术检测Jatamanvaltrate P对T47D细胞凋亡和周期的影响；Western blot检测与细胞周期、凋亡相关蛋白的变化。结果 Jatamanvaltrate P能明显抑制乳腺癌细胞增殖，并且呈时间-剂量依赖性；Jatamanvaltrate P能明显将T47D细胞阻滞于G2/M期，下调周期相关蛋白cdc-2和Cyclin B1，上调p-cdc-2；Jatamanvaltrate P能诱导T47D细胞产生凋亡小体，显著性增加T47D细胞凋亡比例，呈现剂量依赖性；Jatamanvaltrate P处理后的蛋白PARP和Caspase 3/8/9出现了活化形式。结论 Jatamanvaltrate P通过阻滞细胞周期、诱导细胞凋亡来抑制乳腺癌细胞T47D的增殖，有望成为一种潜在的治疗乳腺癌的药物。 |
| 关键词: 蜘蛛香 Jatamanvaltrate P 乳腺癌 细胞周期 凋亡 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.01.003 |
| 分类号:R285.5 |
| 基金项目: |
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| Effect and Mechanism of Jatamanvaltrate P on Proliferation of T47D Cells to Breast Cancer |
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SHEN Weifeng, ZHU Zhihui, ZHU Rui, ZHAO Huajun
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Pharmacy College, Zhejiang Chinese Medical University, Hangzhou 311402, China
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| Abstract: |
| OBJECTIVE To investigate the effect and mechanism of Valeriana jatamansi Jones. extract Jatamanvaltrate P on proliferation of T47D cells to breast cancer. METHODS MTT assay was used to detect the effect of Jatamanvaltrate P on T47D and BT-20 cells; DAPI staining and flow cytometry(FCM) were used to examine cell cycle and apoptosis; Western blot assay was performed to detect the expression of related proteins of cell cycle and apoptosis. RESULTS Jatamanvaltrate P significantly inhibited the breast cancer cells in a time-and dose-dependent; Jatamanvaltrate P induced cell cycle arrest at G2/M phase, and the expression of cycle associated proteins Cyclin B1 and cdc2 was decreased, while p-cdc2 was increased; Jatamanvaltrate P was able to induce T47D cells to produce apoptotic bodies and significantly increase the apoptosis rate of T47D cells in a dose-dependent manner. The proteins of PARP, Caspase 3, Caspase 8 and Caspase 9 produced activated form in T47D cells by treatment with Jatamanvaltrate P. CONCLUSION Jatamanvaltrate P may be possible to inhibit the proliferation of T47D cells by cell cycle arrest and inducing apoptosis, which is expected to become a potential drug for the treatment of breast cancer. |
| Key words: Valeriana jatamansi Jones. Jatamanvaltrate P breast cancer cell cycle apoptosis |
