| 引用本文: | 刘帅英,张晓芹,王慧玉,袁宙新,雷后兴,倪京丽.HPLC同时测定三叶青中5种黄酮类成分的含量[J].中国现代应用药学,2018,35(12):1878-1880. |
| LIU Shuaiying,ZHANG Xiaoqin,WANG Huiyu,YUAN Zhouxin,LEI Houxing,NI Jingli.Simultaneous Determination of Five Major Flavonoids in Tetrastigma Hemsleyanum by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(12):1878-1880. |
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| 摘要: |
| 目的 建立HPLC同时测定三叶青中5个黄酮类化合物芦丁、异槲皮苷、山奈酚-3-O-芸香糖苷、槲皮素和山奈酚的含量。方法 Agilent ZORBAX SB-C18色谱柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.2%磷酸溶液，梯度洗脱，流速为1.0 mL·min-1，检测波长为360 nm，柱温为35℃。结果 芦丁、异槲皮苷、山奈酚-3-O-芸香糖苷、槲皮素和山奈酚分别在0.087 8~0.878 μg、0.069 6~0.696 μg、0.162 9~1.629 μg、0.002 7~0.027 μg、0.007 23~0.072 3 μg内与峰面积呈良好的线性关系，r ≥ 0.999 7；平均加样回收率分别为97.44%，96.88%，97.00%，96.42%，96.24%。结论 该方法简便、高效，可作为三叶青的质量控制提供参考。 |
| 关键词: 三叶青 芦丁 异槲皮苷 山奈酚-3-O-芸香糖苷 槲皮素 山奈酚 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.12.025 |
| 分类号:R917 |
| 基金项目:丽水市科技计划项目（2017ZDYF16） |
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| Simultaneous Determination of Five Major Flavonoids in Tetrastigma Hemsleyanum by HPLC |
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LIU Shuaiying1, ZHANG Xiaoqin2, WANG Huiyu2, YUAN Zhouxin2, LEI Houxing2, NI Jingli2
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1.Lishui Inspection Institute for Food and Drug & Quality and Technology Control, Lishui 323000, China;2.Lishui Hospital of Traditional Chinese Medicines, Lishui 323000, China
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| Abstract: |
| OBJECTIVE To develop a determination method of rutin, isoquercitrin, kaempferol-3-O-rutinoside, quercetin, and kaempferol in Tetrastigma hemsleyanum by HPLC. METHODS HPLC assay was carried out using an Agilent ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm)with column temperature at 35℃. The mobile phase was acetonitrile-0.2% phosphoric acid in gradient elution at a flow rate of 1.0 mL·min-1. And the detection wavelength was 360 nm. RESULTS The linear ranges of rutin, isoquercitrin, kaempferol-3-O-rutinoside, quercetin, kaempferol, and baicalin were 0.087 8-0.878 μg, 0.069 6-0.696 μg, 0.162 9-1.629 μg, 0.002 7-0.027 μg, 0.007 23-0.072 3 μg, respectively (r ≥ 0.999 7), and the average recoveries of three components were 97.44%, 96.88%, 97.00%, 96.42%, 96.24%, respectively. CONCLUSION The method is simple, quick and accurate, can be used for the quality control of the Tetrastigma hemsleyanum. |
| Key words: Tetrastigma hemsleyanum rutin isoquercitrin kaempferol-3-O-rutinoside quercetin kaempferol determination |
