| 引用本文: | 张吟,黄丹丹,张淑芬,许秋霞,李营营.C57BL/6J糖尿病小鼠模型的优化研究[J].中国现代应用药学,2019,36(6):655-660. |
| ZHANG Yin,HUANG Dandan,ZHANG Shufen,XU Qiuxia,LI Yingying.Optimization of C57BL/6J Diabetic Mice Model[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(6):655-660. |
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| 摘要: |
| 目的 优化链脲佐菌素（streptozotocin，STZ）诱导的C57BL/6J糖尿病小鼠模型。方法 采用单用不同剂量STZ（180 mg·kg-1单次给药和275 mg·kg-1均分5次，每次55 mg·kg-1，连续5 d）或4周高脂饮食联合不同剂量STZ（50 mg·kg-1单次给药；100 mg·kg-1单次给药；150 mg·kg-1单次给药；200 mg·kg-1均分2次给药，间隔72 h），建立糖尿病小鼠模型，检测各组小鼠空腹血糖、体质量、日饮水量及日进食量，比较各组造模成功率及稳定性。结果 STZ 180 mg·kg-1单次给药、高脂饮食4周+STZ 150 mg·kg-1单次给药、高脂饮食4周+STZ 200 mg·kg-1均分2次给药得到的糖尿病小鼠模型，其高血糖的持续时间较长且稳定。结论 STZ 180 mg·kg-1单次给药是较为理想的1型糖尿病模型；4周高脂饮食联合STZ 150 mg·kg-1单次给药或200 mg·kg-1均分2次给药均是较为理想的2型糖尿病模型。 |
| 关键词: 糖尿病模型 小鼠 链脲佐菌素 C57BL/6J小鼠 模型优选 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.06.003 |
| 分类号:R965.1 |
| 基金项目:国家卫生计生委共建科学研究基金（WKJ-FJ-13）；福建省卫生系统中青年骨干人才培养项目（2015-ZQN-ZD-23） |
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| Optimization of C57BL/6J Diabetic Mice Model |
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ZHANG Yin1, HUANG Dandan1, ZHANG Shufen1, XU Qiuxia1, LI Yingying2
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1.Department of Pharmacy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China;2.College of Pharmacy, Fujian Medical University, Fuzhou 350108, China
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| Abstract: |
| OBJECTIVE To optimize the C57BL/6J diabetic mice model induced by streptozotocin(STZ). METHODS Different diabetic mice model were established with different doses of STZ intraperitoneal administration (180 mg·kg-1 single dose and 275 mg·kg-1 averaged by 5 d, 55 mg·kg-1 each day) and 4-week high-fat diet combined with different doses of STZ(50 mg·kg-1 single dose, 100 mg·kg-1 single dose, 150 mg·kg-1 single dose and 200 mg·kg-1 for two times at 72 h intervals). Fasting blood glucose, weight, water-drinking and food-consumption were determined, the modeling rate and stability of each group were compared. RESULTS Diabetic model induced by STZ 180 mg·kg-1 single dose, four weeks high-fat diet combined with STZ 150 mg·kg-1 single dose or 200 mg·kg-1 for two times administration were more stable in the long run. CONCLUSION STZ 180 mg·kg-1 single dose is a suitable type 1 diabetic model, meanwhile, four weeks high-fat diet combined with STZ 150 mg·kg-1 single dose or 200 mg·kg-1 for two times administration are appropriate type 2 diabetic model. |
| Key words: diabetic model mice streptozotocin C57BL/6J mice model optimization |
