| 引用本文: | 万睿璇,周抒遥,徐欢笛,汪浩宇,娄云阁,姜净,邹秉杰,宋沁馨,周国华.单细胞基因组和转录组的共测定方法研究进展[J].中国现代应用药学,2018,35(9):1423-1430. |
| WAN Ruixuan,ZHOU Shuyao,XU Huandi,WANG Haoyu,LOU Yunge,JIANG Jing,ZOU Bingjie,SONG Qinxin,ZHOU Guohua.Progress in Parallel Sequencing of the Genomes and Transcriptomes of Single Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(9):1423-1430. |
|
| |
|
|
| 本文已被：浏览 2635次 下载 1572次 |
码上扫一扫！ |
|
|
| 单细胞基因组和转录组的共测定方法研究进展 |
|
万睿璇,周抒遥,徐欢笛,汪浩宇,娄云阁,姜净,邹秉杰,宋沁馨,周国华
|
|
中国药科大学,中国药科大学,中国药科大学,中国药科大学,中国药科大学,中国药科大学,南京军区南京总医院,中国药科大学,南京军区南京总医院
|
|
| 摘要: |
| 单细胞测序提供了大量样品测序所不能提供的异质性等信息。然而由于细胞生长发育受多种因素调节，仅依靠基因型或者表型信息无法全面、准确地说明问题。因此，开发单细胞基因组转录组共测定技术为研究基因变异与表型变异间的关系提供了强有力的工具。目前，单细胞基因组与转录组共测定技术主要分为全细胞裂解法（如：G&T-Seq、DR-Seq），细胞膜裂解法（如：SIDR、微流控芯片法）以及其他方法（如：Simul-Seq、Gel-Seq）。本文对这几类技术进行了简要介绍，并对其前景进行了展望。 |
| 关键词: 多手性中心 手性分析 高效液相色谱法 毛细管电泳 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.09.035 |
| 分类号:R963 |
| 基金项目:国家自然科学基金项目（81673390）；江苏省重点研发计划（社会发展）项目（BE2016745）；江苏省基础研究计划（自然科学基金）项目（BK20151445）；药物质量与安全预警教育部重点实验室资助项目（DQCP2017MS01）；中国药科大学药学基地科研训练及科研能力提高项目（J1310032）；江苏省青蓝工程资助项目 |
|
| Progress in Parallel Sequencing of the Genomes and Transcriptomes of Single Cells |
|
Wan Ruixuan,Zhou Shuyao,Xu Huandi,Wang Haoyu,Lou Yunge,Jiang Jing,Zou Bingjie,Song Qinxin and Zhou Guohua
|
|
China Pharmaceutical University,China Pharmaceutical University,China Pharmaceutical University,China Pharmaceutical University,China Pharmaceutical University,China Pharmaceutical University,Jinling Hospital,China Pharmaceutical University,Jinling Hospital
|
| Abstract: |
| Single-cell sequencing provides information, such as heterogeneity, that is omitted by bulk sample sequencing. However, since cell growth and development are regulated by many factors, relying on genotype or phenotype information alone is not able to comprehensively and accurately explain the problem. Co-detection of single-cell DNA-Seq and RNA-Seq offers a strong tool for researchers on the aspect of the relationship between gene variations and phenotype variations. Up to present, there are strategies described below have been employed:whole cell lysis (e.g.:G&T-Seq, DR-Seq), plasma membrane lysis (e.g.:SIDR, microfluidics platform method) and others (e.g.:Simul-Seq, Gel-Seq). This review will introduce and compare these methods in addition to the prediction of their research trends. |
| Key words: multichiral centers chiral analysis HPLC capillary electrophoresis |
|
|
|
|
