| 引用本文: | 范孟雪,丁斐,秦昆明,杨冰,蔡宝昌.不同品种升麻的HPLC指纹图谱研究[J].中国现代应用药学,2019,36(10):1244-1248. |
| FAN Mengxue,DING Fei,QIN Kunming,YANG Bing,CAI Baochang.Study on HPLC Fingerprints of Different Species of Cimicifuga Rhizoma[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(10):1244-1248. |
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| 不同品种升麻的HPLC指纹图谱研究 |
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范孟雪1, 丁斐2, 秦昆明2,3, 杨冰2,3, 蔡宝昌1,2,3
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1.江苏南中医健康产业研究院有限公司, 南京 210029;2.南京海昌中药集团有限公司, 江苏省企业研究生工作站, 南京 210061;3.南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心, 南京 210023
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| 摘要: |
| 目的 研究3个不同品种升麻的指纹图谱。方法 采用YMC-Pack-ODS-C18（250 mm×4.6 mm，5 μm）色谱柱；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱；流速：1.0 mL·min-1；柱温：30℃；检测波长：320 nm；进样量：10 μL。结果 建立了3个不同品种升麻的指纹图谱，分别鉴别出兴安升麻特征峰17个，大三叶升麻特征峰19个，升麻特征峰23个。结论 该方法重复性好，简单可靠，为升麻药材定性鉴别提供理论基础。 |
| 关键词: 升麻 HPLC 指纹图谱 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.10.015 |
| 分类号:R284.1 |
| 基金项目:江苏省自然科学基金项目（BK20141093） |
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| Study on HPLC Fingerprints of Different Species of Cimicifuga Rhizoma |
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FAN Mengxue1, DING Fei2, QIN Kunming2,3, YANG Bing2,3, CAI Baochang1,2,3
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1.Jiangsu Nanjing University of Chinese Medicine Health Industry Research Institute Co., Ltd., Nanjing 210029, China;2.Nanjing Hydron Chinese Medicine Group Co., Ltd., Enterprise of Jiangsu Province Graduate Workstation, Nanjing 210061, China;3.Nanjing University of Chinese Medicine Processing Standardization and Standardization of Engineering Research Center of National Ministry of Education, Nanjing 210023, China
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| Abstract: |
| OBJECTIVE To study the fingerprints of three different species of Cimicifuga Rhizoma. METHODS YMC-Pack-ODS-C18 column(250 mm×4.6 mm, 5 μm) was taken for study, the mobile phase was acetonitrile-0.1% phosphoric acid solution with gradient elution at the flow rate of was 1.0 mL·min-1, the column temperature was 30℃, the detection wavelength was 320 nm and the injection volume was 10 μL. RESULTS The fingerprints of three different species of Cinicifuga Rhizoma were established. Seventeen characteristic peaks of Cimicifuga dahurica (Turcz.) Maxim, 19 characteristic peaks of Cimicifuga heracleifolia Kom. and 23 characteristic peaks of Cimicifuga foetida L. were pointed out, respectively. CONCLUSION This method is reproducible, simple and reliable, can provide a theoretical basis for the qualitative identification of Cinicifuga Rhizoma. |
| Key words: Cinicifuga Rhizoma HPLC fingerprint |
