| 引用本文: | 王琼芬,刘婷,郑平安,徐虹.GC测定鱼肝油中EPA和DHA含量[J].中国现代应用药学,2018,35(9):1322-1326. |
| WANG Qiongfen,LIU Ting,ZHENG Pingan,XU Hong.Determination of EPA and DHA in Cod-liver Oil by GC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(9):1322-1326. |
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| 摘要: |
| 目的 建立加校正因子GC内标法测定鱼肝油中EPA和DHA含量。方法 样品经皂化、甲酯化、异辛烷提取，采用DB-23（30 m×0.25 mm，0.25 μm）毛细管柱，FID检测器，程序升温（起始温度170℃，以1℃·min-1的速率升温至225℃，保持5 min），进样口温度为250℃，检测器温度为280℃，高纯N2为载气，流速为1.0 mL·min-1，分流比为100∶1，二十三烷酸（C23∶0）甲酯为内标物。结果 EPA、DHA的峰面积比和浓度比分别在0.048 7~9.740 mg·mL-1，0.051 0~10.206 mg·mL-1内呈良好的线性关系（r=1.000 0），EPA、DHA的平均回收率分别为98.7%（RSD=0.98%），99.4%（RSD=1.34%）。结论 该方法简便、准确、重复性好，可用于鱼肝油的质量控制和掺假鉴定。 |
| 关键词: 鱼肝油 EPA DHA 气相色谱法 相对校正因子 内标法 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.09.011 |
| 分类号:R917 |
| 基金项目:浙江省食品药品监管系统科技计划项目（SP201627） |
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| Determination of EPA and DHA in Cod-liver Oil by GC |
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wangqiongfen,liuting,zhengpingan and xuhong
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Zhoushan Institute for Food and Drug Control,Zhoushan Institute for Food and Drug Control,Hailisheng Group Co.Ltd,Zhoushan Institute for Food and Drug Control
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| Abstract: |
| OBJECTIVE To establish a method for the determination of EPA and DHA in cod-liver oil by gas chromatography internal standard method with relative correction factor. METHODS The samples were saponified, methyl esterified and extracted with isooctane. The sample was chromatographed on a DB-23 (30 m×0.25 mm, 0.25 μm) capillary column with a FID detector. The column temperature was raised by program (the initial temperature was 170℃ and the temperature was raised to 225℃ at a rate of 1℃ ·min-1 for 5 min). The injector temperature was 250℃, detector temperature was 280℃, high purity nitrogen was used as carrier gas, flow rate was 1.0 mL·min-1, split flow was 100:1, methyl ester (C23:0) was used as an internal standard. RESULTS EPA and DHA showed good linear relationships (r=1.000 0) with the peak area ratio and concentration ratio at the range of 0.048 7-9.740 mg·mL-1, 0.051 0-10.206 mg·mL-1, respectively. The average recoveries of EPA and DHA were 98.7% (RSD=0.98%), 99.4% (RSD=1.34%). CONCLUSION The method is sample, accurate and reproducible. It can be used for the quality control and adulteration identification of cod-liver oil. |
| Key words: cod-liver oil EPA DHA GC relative correction factor internal standard method determination |
