| 摘要: |
| 目的 建立HPLC双波长法同时测定炎热清片中龙胆苦苷、栀子苷、芒果苷、黄芩苷、黄芩素和汉黄芩素6种成分的含量。方法 采用Agilent TC-C18色谱柱（4.6 mm×250 mm，5 μm），流动相为甲醇∶0.2%磷酸水溶液（梯度洗脱），柱温为35℃，流速为1.0 mL·min-1，检测波长0~20 min为254 nm，同时检测龙胆苦苷、栀子苷、芒果苷，20~50 min为280 nm，同时检测黄芩苷、黄芩素、汉黄芩素。结果 龙胆苦苷、栀子苷、芒果苷、黄芩苷、黄芩素、汉黄芩素分别在18.92~189.2 ng（r=0.999 9），107.44~1 074.4 ng（r=0.999 9），9.82~98.2 ng（r=0.999 9），767.68~7 676.8 ng（r=1.000 0），100.94~1 009.4 ng（r=0.999 9），49.84~498.4 ng（r=0.999 9）线性良好，平均回收率（n=9）分别为98.31%，98.35%，98.40%，98.03%，98.46%，98.24%，RSD分别为0.4%，0.3%，0.3%，0.4%，0.4%，0.3%。结论 该方法灵敏、简便、准确，可用于炎热清片的质量控制。 |
| 关键词: 炎热清片 龙胆苦苷 栀子苷 芒果苷 黄芩苷 黄芩素 汉黄芩素 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.09.013 |
| 分类号:R927.2 |
| 基金项目: |
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| Simultaneous Determination of Six Components in Yanreqing Tablets by Double-wavelength HPLC |
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dengjunjie
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Shaoxing Institute for Food and Drug Control
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| Abstract: |
| OBJECTIVE To establish a new double-wavelength HPLC method for simultaneous determination of 6 main components in Yanreqing tablets. METHODS The method was performed on an Agilent TC-C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was consisited of methanol-0.2% phosphoric acid by gradient elution at 35℃ column temperature. The flow rate was 1.0 mL·min-1. The detection wavelength was 254 nm for gentiopicroside, geniposide, mangiferin at 0-20 min, and 280 nm for baicalin, baicalein, wogonin at 20-50 min. RESULTS Gentiopicroside, geniposide, mangiferin, baicalin, baicalein, wogonin showed a good linear relationship in the range of 18.92-189.2 ng (r=0.999 9), 107.44-1 074.4 ng (r=0.999 9), 9.82-98.2 ng (r=0.999 9), 767.68-7 676.8 ng (r=1.000 0), 100.94-1 009.4 ng (r=0.999 9), 49.84-498.4 ng (r=0.999 9), respectively. The average recoveries were 98.31%, 98.35%, 98.40%, 98.03%, 98.46%, 98.24%, the RSD were 0.4%, 0.3%, 0.3%, 0.4%, 0.4%, 0.3%. CONCLUSION The method is sensitive, simple, accurate, and suitable for the quality control of Yanreqing tablets. |
| Key words: Yanreqing tablets gentiopicroside geniposide mangiferin baicalin baicalein wogonin HPLC |
