| 引用本文: | 邹胜泽,罗哲婵,张雁.一测多评法同时测定正天丸中6种指标性成分[J].中国现代应用药学,2019,36(3):331-335. |
| ZOU Shengze,LUO Zhechan,ZHANG Yan.Determination of Six Index Components in Zhengtian Pills by Quantitative Analysis of Multi-components by Single Marker[J].Chin J Mod Appl Pharm(中国现代应用药学),2019,36(3):331-335. |
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| 摘要: |
| 目的 建立一测多评（quantitative analysis of multi-components by single-marker，QAMS）法同时测定正天丸中6种指标性成分的含量。方法 采用Agilent XDB C18色谱柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.1%磷酸水溶液，梯度洗脱，流速1.0 mL·min-1，检测波长310 nm，柱温30℃，进样量10 μL。应用QAMS法，以芍药苷为参照物，测定6种指标性成分之间的相对校正因子，分别采用外标法和QAMS法测定正天丸中的6种指标性成分的含量，并对2种方法计算结果进行对比分析，以验证QAMS法的实用性与稳定性。结果 建立了用于测定正天丸中6种指标性成分的QAMS法，并对6批正天丸进行测定，其计算值与测定值的差异较小。结论 本研究建立的QAMS法可测定正天丸中6种指标性成分的含量，方法简单、有效，结果准确，可用于正天丸的质量控制，为完善质量标准提供参考。 |
| 关键词: 一测多评法 正天丸 升麻素苷 芍药苷 5-O-甲基维斯阿米醇苷 阿魏酸 欧前胡素 异欧前胡素 |
| DOI：10.13748/j.cnki.issn1007-7693.2019.03.015 |
| 分类号:R284.1 |
| 基金项目: |
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| Determination of Six Index Components in Zhengtian Pills by Quantitative Analysis of Multi-components by Single Marker |
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ZOU Shengze1, LUO Zhechan1, ZHANG Yan2
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1.Wenzhou Hospital of Traditional Chinese Medicine, Wenzhou 325000, China;2.Hunan Xiangtan Institute for Food and Drug Control, Xiangtan 411100, China
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| Abstract: |
| OBJECTIVE To develop a method of quantitative analysis of multi-components by single marker(QAMS) for six index components in Zhengtian pills. METHODS The method with a Agilent XDB C18 column(250 mm×4.6 mm, 5 μm) was applied, the mobile phase was acetonitrile-0.1% phosphoric acid of gradient elution, the flow rate was 1.0 mL·min-1,the detection wavelength was 310 nm,the column temperature was 30℃ and the injection volume was 10 μL. Used paeoniflorin as a reference, the relative correction factors among the six kinds index components were detected by QAMS. These six index contents were determined by external standard method and QAMS. The results obtained from two different methods were compared. Verified the practicability and stability of QAMS. RESULTS The established QAMS was used to determine the six index components in Zhengtian pills, and totally six batches of Zhengtian pills were determined. There was no significant difference in the calculated values and the measured values. CONCLUSION The QAMS method is simple, effective and accurate in determining the contents of six index components in Zhengtian pills, which can be used for the quality control of Zhengtian pills and provide reference for the improvement of quality standards. |
| Key words: quantitative analysis of multi-components by single marker Zhengtian pills prim-O-glucosylcimifugin paeoniflorin 5-O-methylvisammioside ferulic acid imperatorin isoimperatorin |
