| 引用本文: | 陆静娴,施贝,董婷,陈碧莲,马临科,祝明.UHPLC同时测定进口西洋参4个化学成分的含量[J].中国现代应用药学,2018,35(10):1443-1446. |
| LU Jingxian,SHI Bei,DONG Ting,CHEN Bilian,MA Linke,ZHU Ming.Simultaneous Determination of Four Constituents in Imported Panax Quinquefolium L. by UHPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(10):1443-1446. |
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| UHPLC同时测定进口西洋参4个化学成分的含量 |
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陆静娴,施贝,董婷,陈碧莲,马临科,祝明
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浙江省食品药品检验研究院,浙江省食品药品检验研究院,浙江省食品药品检验研究院,浙江省食品药品检验研究院,浙江省食品药品检验研究院,浙江省食品药品检验研究院
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| 摘要: |
| 目的 建立UHPLC同时测定进口西洋参中4个活性成分（人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1和人参皂苷Rd）含量的方法。方法 采用Zorbax SB C18（2.1 mm×100 mm，1.8 μm）色谱柱，以乙腈为流动相A，水为流动相B，梯度洗脱，流速0.5 mL·min-1，柱温30℃，检测波长为203 nm。结果 4种成分的分离度良好，且在各自浓度范围内呈现良好的线性关系（r≥0.999 9），平均回收率为95.7%~100.3%（RSD1.4%~1.8%）。结论 该方法操作简单，缩短了分析时间，重复性好，为评价进口西洋参的质量提供了可靠的方法。 |
| 关键词: 西洋参 超高效液相色谱法 人参皂苷Rg1 人参皂苷Re 人参皂苷Rb1 人参皂苷Rd |
| DOI：10.13748/j.cnki.issn1007-7693.2018.10.002 |
| 分类号:R917 |
| 基金项目:浙江省中医药科技计划项目（2016ZB081） |
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| Simultaneous Determination of Four Constituents in Imported Panax Quinquefolium L. by UHPLC |
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LU Jing-xian,SHI Bei,DONG Ting,CHEN Bi-lian,MA Lin-ke and ZHU Ming
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Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control,Zhejiang Institute for Food and Drug Control
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| Abstract: |
| OBJECTIVE To establish a UHPLC method for simultaneous determination of four active components (ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd) in imported Panax quinquefolium L.. METHODS An Agilent Zorbax SB C18 column (2.1 mm×100 mm, 1.8 μm) was used, with mobile phase of acetonitrile as mobile phase A and water as mobile phase B (gradient elution) at a flow rate of 0.5 mL·min-1, column temperature was 30℃ and detector wavelength was 203 nm. RESULTS The four components showed good separation and linearity within the tested range. The mean recoveries were in the range of 95.7%-100.3% (RSD 1.4%-1.8%). CONCLUSION It provides a rapid and reliable UHPLC method which is suitable for the quality control of Panax quinquefolium L.. |
| Key words: Panax quinquefolium L. UHPLC ginsenoside Rg1 ginsenoside Re ginsenoside Rb1 ginsenoside Rd |
