| 引用本文: | 姚晓,蒋骊龙,陈建伟.双波长HPLC同时测定小儿咳喘灵颗粒中8种有效成分的含量[J].中国现代应用药学,2018,35(7):1036-1040. |
| YAO Xiao,JIANG Lilong,CHEN Jianwei.Simultaneous Determination of Eight Effective Components in Child Kechuanling Granules by Double Wavelength HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(7):1036-1040. |
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| 摘要: |
| 目的 采用HPLC同时测定小儿咳喘灵颗粒中盐酸麻黄碱、盐酸伪麻黄碱、（R，S）告依春、绿原酸、苦杏仁苷、甘草苷、木犀草苷、甘草酸的含量。方法 采用双波长HPLC，以Agilent ZORBAX SB-C18色谱柱（4.6 mm×250 mm，5 μm）进行分离；流动相：0.1%磷酸水溶液-乙腈梯度洗脱；流速1 mL·min-1；柱温30℃；检测波长210 nm（盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷、甘草苷、木犀草苷），245nm[（R，S）告依春、绿原酸、甘草酸]。结果 8种成分的峰面积与质量浓度的线性关系良好，加样回收率96.23%~100.7%。结论 该方法简便、快速、准确，可用于小儿咳喘灵颗粒的质量控制。 |
| 关键词: 小儿咳喘灵颗粒 盐酸麻黄碱 盐酸伪麻黄碱 (R,S)告依春 双波长 高效液相色谱法 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.07.020 |
| 分类号:R927.2 |
| 基金项目:江苏高校优势学科建设工程资助项目（YSXK-2010） |
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| Simultaneous Determination of Eight Effective Components in Child Kechuanling Granules by Double Wavelength HPLC |
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YAO Xiao1, JIANG Lilong2, CHEN Jianwei3
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1.Changzhou Food and Drug Administration, Changzhou 213002, China;2.China Pharmaceutical University, Nanjing 211198, China;3.College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for determine the contents of ephedrine hydrochloride, pseudoephedrine hydrochloride, epigoitrin, chlorogenic acid, amygdalin, liquiritin, luteoloside and glycyrrhetic acid in Child Kechuanling granules at the same time. METHODS A double wavelength HPLC method was performed. The chromatographic separations were achieved on a Agilent ZORBAX SB-C18 column(4.6 mm×250 mm, 5 μm). The mobile phase of acetonitrile and 0.1% phosphoric acid solution was used with a gradient elution.The flow rate was 1.0 mL·min-1 and the column temperature was 30℃. The detector wavelength was 210 nm for ephedrine hydrochloride, pseudoephedrine hydrochloride, amygdalin, liquiritin and luteoloside and 245 nm for (R,S)-goitrin, chlorogenic acid and glycyrrhetic acid. RESULTS Eight constituents were seperated perfectly and good linear relationships were obtained. The recoveries were between 96.23%-100.7%. CONCLUSION The method is reliable, simple and accurate, and can be used for the quality control of Child Kechuanling granules. |
| Key words: Child Kechuanling granules ephedrine hydrochloride pseudoephedrine hydrochloride (R,S)-goitrin double wavelength HPLC content determination |
