| 引用本文: | 顾利强,章媛,徐晓珍,陈灵芳,张升,黄敏聪,辛艳飞,郑高利,陈忠坚.大鼠脑脊液多次采集改良模型的建立[J].中国现代应用药学,2018,35(3):311-313. |
| Gu Liqiang,Zhang Yuan,Xu Xiaozhen,Chen Lingfang,Zhang Sheng,Huang Mincong,Xin Yanfei,Zheng Gaoli,Chen Zhongjian.Establishment of Cerebrospinal Fluid Multiple Collection Model in Rat[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(3):311-313. |
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| 大鼠脑脊液多次采集改良模型的建立 |
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顾利强1,2, 章媛3, 徐晓珍1, 陈灵芳1, 张升1, 黄敏聪1, 辛艳飞1, 郑高利1, 陈忠坚4
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1.浙江省医学科学院安全性评价研究中心, 杭州 310013;2.浙江中医药大学药学院, 杭州 310053;3.杭州市萧山区第三人民医院, 杭州 311251;4.浙江省肿瘤医院肿瘤研究所, 杭州 310022
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| 摘要: |
| 目的 建立大鼠多次采集脑脊液的动物模型，以进行药物在大鼠脑部的动力学研究。方法 将大鼠麻醉后，利用脑立体定位仪，通过大鼠双侧耳道与上方门齿固定大鼠头部，切开大鼠头部皮肤，暴露颅骨与颈部肌肉交界处，将采样针对准大鼠颅骨与颈部肌肉交界处的中心位置，利用脑立体定位仪的上下轴将采样针竖直缓慢向下刺入，刺入约0.6~0.9 cm（视大鼠体质量而定）后，脑脊液即可被采出。结果 脑脊液采集成功率（脑脊液成功采样1次及以上）100%（26例），动力学采样成功率（脑脊液成功采样6次及以上）80%（10例）。结论 本模型操作简单、结果稳定、成功率高、重复性高，适合脑部作用药物进行脑脊液动力学研究。 |
| 关键词: 大鼠 脑脊液 采集模型 脑部药动学 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.03.002 |
| 分类号:R965.1 |
| 基金项目:国家自然科学基金（81302840）；浙江省医药卫生科技计划科研基金项目（2017KY305）；浙江省科技计划项目（2016F10007）；浙江省医学科学院安评中心科研基金项目（ZA1701S） |
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| Establishment of Cerebrospinal Fluid Multiple Collection Model in Rat |
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Gu Liqiang1,2, Zhang Yuan3, Xu Xiaozhen1, Chen Lingfang1, Zhang Sheng1, Huang Mincong1, Xin Yanfei1, Zheng Gaoli1, Chen Zhongjian4
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1.Center of Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China;2.College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China;3.The third people's Hospital of Xiaoshan District, Hangzhou 311251, China;4.Zhejiang Cancer Research Institute, Zhejiang Cancer Hospital, Hangzhou 310022, China
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| Abstract: |
| OBJECTIVE To study the pharmacokinetics of drug in rat's brain by establishing a model that can collect cerebrospinal fluid at different times. METHODS The rat's head was fixed by the antrum auris and cutting tooth after anesthesia using brain stereotaxis. Cut and open the skin in corona capitis to find the crossing line between the cranium and muscle in cervical part. The needle used to collect the cerebrospinal fluid pricked into the center point of the crossing line and was pushed down by the "Z" axis slowly. When the depth of 0.6-0.9 cm (the number of the depth depended on the body weight of the rat) was arrived, the cerebrospinal fluid would be got. RESULTS The achievement ratio of single collection was 100% based on the data of 26 rats and the achievement ratio of multiple collections (6 times and above) was 80% based on the data of 10 rats. CONCLUSION The model is proved to be simple, stable, high efficient and repeatable and is suitable to be adopted to apply in brain research in rats. |
| Key words: rat cerebrospinal fluid collection model brain pharmacokinetics |
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