| 引用本文: | 余乐,刘敏,陈张金,王伟影.浙产三叶青的质量控制研究[J].中国现代应用药学,2018,35(8):1194-1198. |
| YU Le,LIU Min,CHEN Zhangjin,WANG Weiying.Quality Control of the Tetrastigmae Hemsleyanum from Zhejiang Province[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(8):1194-1198. |
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| 摘要: |
| 目的 探讨制定浙产三叶青质量控制的方法和限度。方法 运用显微鉴别法、化学反应鉴别法进行鉴别，采用UV分别对活性成分（总黄酮、总多糖、总氨基酸）进行测定；参照中国药典2015年版四部相关方法对水分、总灰分、重金属及有害元素和醇溶性浸出物进行测定。结果 浙产三叶青的化学反应、显微特征明显；12批样品的测定结果表明，总黄酮、总多糖和总氨基酸的含量分别为0.18%~0.66%，21.59%~61.76%，0.93%~3.68%；水分、总灰分和醇溶性浸出物分别为7.6%~14.3%，2.2%~5.7%和12.4%~22.6%；铅、镉、砷、汞、铜均未超过中国药典2015年版已收载品种重金属及有害元素项下的限量标准。结论 研究所得方法简便、准确，可用于浙产三叶青的质量控制。 |
| 关键词: 浙产三叶青 紫外分光光度法 重金属 有害元素 鉴别 质量控制 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.08.017 |
| 分类号:R284.1 |
| 基金项目:丽水市科技计划项目（2015sjzc07） |
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| Quality Control of the Tetrastigmae Hemsleyanum from Zhejiang Province |
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YU Le,LIU Min,CHEN Zhangjin and WANG Weiying
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Lishui Institute of Food and Drug ＆ Quality and Technical Inspection;Zhejiang,China,Lishui Institute of Food and Drug ＆ Quality and Technical Inspection;Zhejiang,China,Lishui Institute of Food and Drug ＆ Quality and Technical Inspection;Zhejiang,China,Lishui Institute of Food and Drug ＆ Quality and Technical Inspection;Zhejiang,China
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| Abstract: |
| OBJECTIVE To establish quality control methods and limitation of Tetrastigmae hemsleyanum from Zhejiang province. METHODS Microscopical chatacteristics and chemical reaction were used to identify, and UV was used for determining its active ingredients(total flavonoids, total polysaccharides and total amino acids). The water content, total ash, heavy metals, harmful elements and ethanol-soluble extract were determined according to the appendix of China Pharmacopoeia (2015 Edition). RESULTS Characteristics of chemical reaction and microscopical identification were obvious. The contents of total flavonoids, total polysaccharides and total amino acids in 12 batches of Tetrastigmae hemsleyanum from Zhejiang province were in the range of 0.18%-0.66%, 21.59%-61.76% and 0.93%-3.68%, respectively. The results of water content, total ash and ethanol-soluble extract were in the range of 7.6%-14.3%, 2.2%-5.7% and 12.4%-22.6%. The content of Pb, Cd, As, Hg and Cu didn't exceed the limit standards of heavy metals and harmful elements which had been collected in the China Pharmacopoeia(2015 Edition). CONCLUSION This method is simple, accurate and applicable for the quality control of Tetrastigmae hemsleyanum from Zhejiang province. |
| Key words: Tetrastigmae hemsleyanum from Zhejiang Province utraviolet spectroscopy heavy metals harmful elements identification quality control |
