| 引用本文: | 张瑞堂,金永新,张红梅,马肖,何彩虹,康玉龙.镇癫开窍颗粒提取工艺研究[J].中国现代应用药学,2018,35(5):660-664. |
| ZHANG Ruitang,JING Yongxin,ZHANG Hongmei,MA Xiao,HE Caihong,KANG Yulong.Study on Extraction Process of Zhendian Kaiqiao Granules[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(5):660-664. |
|
| 摘要: |
| 目的 确定镇癫开窍颗粒的最佳提取工艺。方法 应用HPLC同时测定龙胆苦苷、芍药苷、黄芩苷的含量，并以含量测定的综合评分为考察指标，采用正交试验法L9（34）优选提取工艺条件。结果 对提取工艺的影响因素从大到小依次为提取次数、提取时间、乙醇浓度、加入溶媒量，最适工艺条件为8倍量的80%乙醇，浸泡30 min，提取3次，每次60 min。结论 该工艺稳定、可行。 |
| 关键词: 镇癫开窍颗粒 龙胆苦苷 芍药苷 黄芩苷 提取工艺 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.05.009 |
| 分类号:R284.2 |
| 基金项目:中央高校基本科研业务费专项项目（31920150095） |
|
| Study on Extraction Process of Zhendian Kaiqiao Granules |
|
ZHANG Ruitang1,2, JING Yongxin2, ZHANG Hongmei2, MA Xiao2, HE Caihong2, KANG Yulong2
|
|
1.Northwest University for Nationatlities, Lanzhou 730000, China;2.Gansu Province of Second People's Hospital, Lanzhou 730000, China
|
| Abstract: |
| OBJECTIVE To optimum the extraction procedure of Zhendian Kaiqiao granules.METHODS Gentiopicrin, paeoniflorin and baicalin were determined by HPLC, the extraction ratio of the content of gentiopicrin, paeoniflorin and baicalin were used as index. The optimum condition of the extraction were study by the orthogonal L9(34).RESULTS The influencing effects of factors were as follows:D > B > A > C (A:alcohol concentration; B:bextraction time; C:the amount of vehicle; D:extraction times). The optimize extraction condition were as follows:8 times of 80% alcohol, soaking 30 min, reflux 60 min, extract 3 times.CONCLUSION The optimum extraction procedure is stable and practical. |
| Key words: Zhendian Kaiqiao granules gentiopicrin paeoniflorin baicalin extract technology HPLC |
