| 引用本文: | 迪里木拉提·毛里明,郭玉娟,程煜凤,任丽君,姚军.阿尔泰锦鸡儿不同药用部位主要黄酮苷的含量测定[J].中国现代应用药学,2018,35(5):704-707. |
| DILIMULATI Maoliming,GUO Yujuan,CHENG Yufeng,REN Lijun,YAO Jun.Study on Content Determination of Main Flavonoid Glycosides in Different Medical Parts of Caragana Altaica Kom. Pojark[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(5):704-707. |
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| 摘要: |
| 目的 测定采集于新疆阿勒泰地区的10批次阿尔泰锦鸡儿根、茎、叶、花与果实药材中芦丁和山奈酚-3-O-芸香糖苷的含量。方法 色谱条件：Inertsil ODS-3色谱柱（250 mm×4.6 mm，5 μm），检测波长254 nm，流速1.0 mL·min-1，进样量5 μL，柱温25℃，流动相为0.2%磷酸（A）-乙腈（B），线性梯度洗脱（0~5 min，10%→14% B；5~10 min，14%→17% B；10~16 min，17%→20% B；16~21 min，20%→22% B；21~30 min，22%→25% B；30~40 min，25% B）。结果 10批次阿尔泰锦鸡儿叶、花与果实药材中芦丁的平均含量为：果实6.83 mg·g-1，叶6.16 mg·g-1，花3.58 mg·g-1；山奈酚-3-O-芸香糖苷的平均含量为：果实1.48 mg·g-1，叶2.44 mg·g-1，花1.01 mg·g-1。根与茎供试品溶液中均未检测到这2种黄酮苷成分。结论 建立的方法简便、稳定性、重复性良好，为阿尔泰锦鸡儿药材黄酮类成分的研究提供了实验基础，亦为建立该药材的质量标准提供了重要的实验数据。 |
| 关键词: 阿尔泰锦鸡儿 不同药用部位 黄酮苷 芦丁 山奈酚-3-O-芸香糖苷 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.05.018 |
| 分类号:R284.1;R971.101 |
| 基金项目:新疆地产中药民族药新药研发培育项目（2017-02-03） |
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| Study on Content Determination of Main Flavonoid Glycosides in Different Medical Parts of Caragana Altaica Kom. Pojark |
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DILIMULATI Maoliming1, GUO Yujuan1, CHENG Yufeng1, REN Lijun2, YAO Jun1
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1.College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China;2.Xinjiang Changji Vocational and Technical College, Changji 831100, China
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| Abstract: |
| OBJECTIVE To determine the content of rutin and kaempferol-3-O-rutinoside in the roots, stems, leaves, flowers and fruits of Caragana altaica kom. pojark from Xinjiang Altai Region.METHODS HPLC conditions:Inertsil ODS-3 column(250 mm×4.6 mm, 5 μm), detection wavelength at 254 nm, flow rate at 1.0 mL·min-1, injection volume was 5 μL and column temperature was 25℃. A gradient elution of 0.2% phosphoric acid (A) and acetonitrile (B) solution were performed with following procedure:0-5 min, 10%→14% B; 5-10 min, 14%→17% B; 10-16 min, 17%→20% B; 16-21 min, 20%→22% B; 21-30 min, 22%→25% B; 30-40 min, 25% B.RESULTS The average content of rutin in 10 batches of fruits, leaves and flowers was 6.83, 6.16, 3.58 mg·g-1 and the average content of kaempferol-3-O-rutinoside was 1.48, 2.44, 1.01 mg·g-1, respectively. However, neither rutin nor kaempferol-3-O-rutinoside were detected in the roots and stems of Caragana altaica kom. pojark.CONCLUSION The content determination method established in this study is simple and showes good stability and repeatability. Furthermore, this study provides experimental basis for the study of flavonoid glycosides in Caragana altaica kom. pojark. and provides essential experimental data for the establishment of quality standard of that plant. |
| Key words: Caragana altaica kom.pojark different medical parts flavonoid glycosides rutin kaempferol-3-O-rutinoside content determination |
