| 引用本文: | 张小林,张文静,陈腊,蒋峰,陈忠波,张行泉.脑胶质瘤中单酰甘油脂肪酶的表达及意义[J].中国现代应用药学,2018,35(6):905-907. |
| ZHANG Xiaolin,ZHANG Wenjing,CHEN La,JIANG Feng,CHEN Zhongbo,ZHANG Xingquan.Expression and Significance of Monoacylglycerol Lipase in Brain Glioma[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(6):905-907. |
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| 摘要: |
| 目的 研究内源性大麻素代谢酶单酰甘油脂肪酶（monoacylglycerol lipase，MAGL）在脑胶质瘤组织中的表达情况及其与胶质瘤病理级别的关系，探讨其临床意义。方法 选取脑胶质瘤标本23例，其中WHO Ⅰ级1例，WHO Ⅱ级7例，WHO Ⅲ级5例，WHO Ⅳ级10例。对照组8例，均为脑挫裂伤后行内减压术切除的正常脑组织。应用实时荧光定量PCR（qRT-PCR）、LC-MS分别检测各组织标本中MAGL mRNA、MAGL活性的表达。结果 MAGL mRNA、MAGL活性在低级别（WHOⅠ~Ⅱ级）和高级别（WHOⅢ~Ⅳ级）脑胶质瘤组织中的表达水平较正常脑组织明显降低（P<0.01），且随着肿瘤病理级别的增高而降低，与肿瘤的病理分级呈负相关（均P<0.01）。MAGL mRNA的表达水平与MAGL活性呈正相关。结论 MAGL可能参与了脑胶质瘤的发生、发展过程，检测MAGL的表达可评价脑胶质瘤的恶性程度。 |
| 关键词: 大麻素 2-花生四烯酸甘油 单酰甘油脂肪酶 胶质瘤 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.06.026 |
| 分类号:R965 |
| 基金项目: |
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| Expression and Significance of Monoacylglycerol Lipase in Brain Glioma |
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ZHANG Xiaolin, ZHANG Wenjing, CHEN La, JIANG Feng, CHEN Zhongbo, ZHANG Xingquan
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Zhejiang Province Zhoushan Hospital, Zhoushan 316021, China
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| Abstract: |
| OBJECTIVE To investigate the relationship between the expression of endocannabinoid metabolic enzyme monoacylglycerol lipase (MAGL) in human brain glioma tissues and the grade of brain glioma, explore its clinicopathological significance. METHODS twenty-three patients with brain glioma, including one case with WHO grade Ⅰ, 7 cases with WHO grade Ⅱ, 5 cases with WHO grade Ⅲ, and 10 cases with WHO grade Ⅳ, performing surgical excision were enrolled in the study and 8 samples of normal brain tissues from patients performing decompression operation due to cerebral hernia served as controls. qRT-PCR and LC-MS were used to detect the mRNA and activity of MAGL. RESULTS The mRNA expression and activity of MAGL was significantly higher in low grade (WHO grade Ⅰ-Ⅱ) and high grade (WHO grade Ⅲ-Ⅳ) brain glioma tissues than those in the normal brain tissues, and both were negatively correlated to the pathological grade (P<0.01). the MAGL mRNA expression was positively correlated with the the MAGL activity. CONCLUSION MAGL may be involved in the occurrence and development of brain glioma. Detection of MAGL expression may be can evaluate the matignant of brain gliomas. |
| Key words: cannabinoid 2-arachidonylglycerol monoacylglycerol lipase glioma |
