| 摘要: |
| 目的 探讨依普利酮对糖尿病肾病（diabetic nephropathy，DN）大鼠的保护机制。方法 DN模型大鼠随机分成模型组、依普利酮组（40 mg·kg-1）、阳性对照组（缬沙坦，20 mg·kg-1），另设正常组。灌胃给药8周。全自动生化分析仪检测24 h尿蛋白、血肌酐及血糖化血红蛋白水平；HE染色行肾组织病理形态学观察；ELISA检测肾组织IL-6、TNF-α和MCP-1水平；qPCR检测TLR4、NF-κB p65 mRNA水平，Western blot法检测肾组织TLR4、NF-κB p65蛋白水平。结果 与模型组比较，依普利酮组大鼠24 h尿蛋白、血肌酐及血糖化血红蛋白水平均明显下降（P<0.05或P<0.01）；肾组织病理变化有不同程度的改善；肾组织IL-6、TNF-α和MCP-1水平均明显下降（P<0.05或P<0.01），TLR4、NF-κB p65 mRNA水平和TLR4、NF-κB p65蛋白水平明显下降（P<0.01）。结论 依普利酮能有效地改善DN大鼠的炎症水平，机制可能与下调IL-6、TNF-α、MCP-1、TLR4、NF-κB p65水平有关。 |
| 关键词: 依普利酮 糖尿病肾病 炎性因子 TLR4 NF-κB p65 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.06.006 |
| 分类号:R965.2 |
| 基金项目: |
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| Protective Mechanism of Eplerenone on Rats with Diabetic Nephropathy |
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LIU Shuwei
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Henan Medical College Hospital Workers, Xinzheng 451191, China
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| Abstract: |
| OBJECTIVE To study the protective mechanism of eplerenone on rats with diabetic nephropathy (DN). METHODS DN model rats were randomly assigned as follows:model group, eplerenone group (40 mg·kg-1) and positive control group (valsartan, 20 mg·kg-1). And a normal group was set. The drugs were given to rats daily for 8 weeks by instragastric administration. The 24 h urinary protein, serum creatinine and the blood glucose hemoglobin levels were measured by automatic biochemical analyzer. Then observed the levels of IL-6, TNF-α and MCP-1 changs in renal tissue by ELISA. The mRNA expression of TLR4, NF-κB p65 in renal tissue were examined by qPCR, the protein expression of TLR4, NF-κB p65 in renal tissue were examined by Western blot. RESULTS Compared with the model group, the levels of 24 h urinary protein, serum creatinine and glycosylated hemoglobin in eplerenone group were significantly decreased (P<0.05 or P<0.01);the pathological changes of renal tissue were improved in different degree;the levels of IL-6, TNF-α and MCP-1 in renal tissue of rats in eplerenone group were decreased significantly (P<0.05 or P<0.01);the mRNA level of TLR4, NF-κB p65 and the protein level of TLR4, NF-κB p65 in renal tissue of rats with eplerenone was also decreased significantly (P<0.01). CONCLUSION Eplerenone can effectively improve the inflammation level of rats with DN, which may be related with decreasing IL-6, TNF-α, MCP-1, TLR4, NF-κB p65 levels. |
| Key words: eplerenone diabetic nephropathy inflammatory factor TLR4 NF-κB p65 |
