| 引用本文: | 冯炎林,田伟强,蒋晓梅.UPLC-MS/MS检测大鼠体内达卢生坦的血药浓度及其药动学研究[J].中国现代应用药学,2018,35(4):506-509. |
| Feng Yanlin,Tian Weiqiang,Jiang xiaomei.Study on Plasma Concentration and Pharmacokinetics of Darusentan in Rat Plasma by UPLC-MS/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(4):506-509. |
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| 摘要: |
| 目的 建立一种快速、高灵敏度和高选择性的UPLC-MS/MS用于大鼠血浆中达卢生坦的测定。方法 通过蛋白沉淀法完成血浆样品处理。通过Acquity UPLC BEH C18柱（2.1 mm×50 mm，1.7 μm）色谱柱，流动相为0.1%甲酸水-乙腈，流速为0.4 mL·min-1。采用ESI+方式检测，扫描方式为多反应离子监测方式检测。结果 达卢生坦血药浓度在0.01~2.5 μg·mL-1内线性关系良好（R2>0.999 6），定量下限（LLOQ）为0.01 μg·mL-1。血浆中的达卢生坦平均回收率为87.613%~97.115%，日内及日间精密度RSD<7.71%，基质效应RSD<9.34%。结论 该方法灵敏高，快速高效，能成功应用于大鼠口服10.0 mg·kg-1达卢生坦后的药动学研究。 |
| 关键词: 达卢生坦 UPLC-MS/MS 大鼠血浆 药动学 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.04.011 |
| 分类号:R917.101 |
| 基金项目: |
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| Study on Plasma Concentration and Pharmacokinetics of Darusentan in Rat Plasma by UPLC-MS/MS |
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Feng Yanlin, Tian Weiqiang, Jiang xiaomei
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Department of phmarcy, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui Municipal Central Hospital, Lishui 323000, china
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| Abstract: |
| OBJECTIVE To develop a rapid, highly sensitive and selective UPLC-MS/MS for the determination of darusentan in rat plasma. METHODS Plasma samples were processed by protein precipitation. The separation was achieved by a gradient mobile phase, consisting of an aqueous solution of 0.1% formic acid and acetonitrile using an Acquity UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm). The flow rate was 0.4 mL·min-1. ESI+ was used to detect. The scanning mode was MRM. RESULTS The standard curve was linear over the range of 0.01-2.5 μg·mL-1(R2>0.999 6) with a LLOQ of 0.01 μg·mL-1. The average recovery of darusentan in plasma was 87.613%-97.115%. The RSD of intra-and inter-assay precision andaccuracy were <7.71%. The RSD of matrix effect was <9.34%. CONCLUSION This method is successfully applied in pharmacokinetic study after oral administration of 10.0 mg·kg-1 darusentan in rats. |
| Key words: darusentan UPLC-MS/MS rat plasma pharmacokinetic |
