| 引用本文: | 黄文康,岳超,宋剑锋,丁国琴,张文婷,赵维良.HPLC同时测定衢枳壳中7种指标成分的含量[J].中国现代应用药学,2018,35(3):404-407. |
| HUANG Wenkang,YUE Chao,SONG Jianfeng,DING Guoqin,ZHANG Wenting,ZHAO Weiliang.Simultaneous Determination of Seven Constituents in Citrus changshanhuyou Y. B. Chang by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(3):404-407. |
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| HPLC同时测定衢枳壳中7种指标成分的含量 |
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黄文康1, 岳超2, 宋剑锋3, 丁国琴4, 张文婷2, 赵维良2
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1.浙江中医药大学, 杭州 310053;2.浙江省食品药品检验研究院, 杭州 310052;3.衢州市食品药品检验研究院, 浙江 衢州 324000;4.杭州市中医院, 杭州 310007
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| 摘要: |
| 目的 建立HPLC同时测定衢枳壳中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、木犀草素、川陈皮素和桔皮素含量。方法 采用Agilent Extend C18（4.6 mm×250 mm，5 μm）色谱柱，检测波长：330 nm，以乙腈-0.1%甲酸溶液为流动相，梯度洗脱，流速：1 ml·min-1，柱温：30℃。结果 芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、木犀草素、川陈皮素、桔皮素分别在23.30~1 164.80 ng，170.84~8 541.91 ng，17.22~861.20 ng，156.17~7 808.64 ng，3.13~156.48 ng，0.90~45.23 ng，0.85~42.27 ng内线性关系良好（r ≥ 0.999 5，n=6），平均回收率分别为98.8%，101.3%，98.3%，96.8%，101.8%，101.7%，108.9%。结论 首次建立了衢枳壳中7个成分HPLC含量测定方法，该方法简便，结果准确，可为综合评价衢枳壳质量提供依据。 |
| 关键词: 衢枳壳 高效液相色谱法 芸香柚皮苷 柚皮苷 橙皮苷 新橙皮苷 木犀草素 川陈皮素 桔皮素 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.03.021 |
| 分类号:R917.101 |
| 基金项目: |
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| Simultaneous Determination of Seven Constituents in Citrus changshanhuyou Y. B. Chang by HPLC |
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HUANG Wenkang1, YUE Chao2, SONG Jianfeng3, DING Guoqin4, ZHANG Wenting2, ZHAO Weiliang2
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1.Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China;3.Quzhou Institute for Food and Drug Control, Quzhou 324000, China;4.Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou 310007, China
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| Abstract: |
| OBJECTIVE To establish a method for the simultaneous determination of the contents of narirutin, naringin, hesperidin, neohesperidin, luteolin, nobiletin and hesperetin of Citrus changshan-huyou Y.B.Chang by HPLC. METHODS The HPLC method was adopted with Agilent Extend C18 column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile and 0.1% methanoic acid with gradient elution. The flow rate was 1 mL·min-1. The detection wavelength was 330 nm and column temperature was 30℃. RESULTS The linear relation of narirutin, naringin, hesperidin, neohesperidin, luteolin, nobiletin and hesperetin was excellent within the range of 23.30-1 164.80 ng, 170.84-8 541.91 ng, 17.22-861.20 ng, 156.17-7 808.64 ng, 3.13-156.48 ng, 0.90-45.23 ng, 0.85-42.27 ng (r ≥ 0.999 5, n=6), and the mean recovery was 98.8%, 101.3%, 98.3%, 96.8%, 101.8%, 101.7%, 108.9%. CONCLUSION It is the first time to establish the HPLC method of the simultaneous determination for the seven contents of Citrus changshan-huyou Y.B.Chang. The method is simple, reliable and accurate, which provides the reference for the quality control for Citrus changshan-huyou Y.B.Chang. |
| Key words: Citrus changshan-huyou Y.B.Chang HPLC narirutin naringin hesperidin neohesperidin luteolin nobiletin hesperetin |
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