| 引用本文: | 王文鹏,李响明,周永妍,冯玉康,郝鹏彬,高亮,孙胜斌,姜国志.HPLC同时测定丹绿补肾胶囊中次野鸢尾黄素和胡椒碱的含量[J].中国现代应用药学,2018,35(7):1001-1003. |
| WANG Wenpeng,LI Xiangming,ZHOU Yongyan,FENG Yukang,HAO Pengbin,GAO Liang,SUN Shengbin,JIANG Guozhi.Simultaneous Determination of Irisflorentin and Piperine in Danlyu Bushen Capsules by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(7):1001-1003. |
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| HPLC同时测定丹绿补肾胶囊中次野鸢尾黄素和胡椒碱的含量 |
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王文鹏1, 李响明1, 周永妍1, 冯玉康1, 郝鹏彬1, 高亮1, 孙胜斌1, 姜国志2
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1.神威药业集团有限公司, 石家庄 051430;2.中药注射剂新药技术开发国家地方联合工程实验室, 石家庄 051430
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| 摘要: |
| 目的 建立同时测定丹绿补肾胶囊中次野鸢尾黄素、胡椒碱含量的HPLC方法。方法 选用Agilent ZORBAX Eclipse Plus C18色谱柱（4.6 mm×250 mm，5 μm），乙腈-0.2%磷酸为流动相（41∶59），流速为1.0 mL·min-1，柱温为40℃，检测波长为266 nm。结果 次野鸢尾黄素和胡椒碱分别在0.010 30~0.309 0 μg，0.081 73~2.451 9 μg内呈良好的线性关系，相关系数≥0.999 8；加样回收率（n=9）分别为99.7%，100.6%；RSD分别为1.2%，0.9%；样品在24 h内稳定，次野鸢尾黄素和胡椒碱RSD分别为1.6%，0.6%；在测定样品中，次野鸢尾黄素和胡椒碱最低含量分别为每粒0.35，6.4 mg。结论 该方法简便、准确、重复性好，可作为丹绿补肾胶囊的质量控制方法。 |
| 关键词: 丹绿补肾胶囊 次野鸢尾黄素 胡椒碱 高效液相色谱法 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.07.012 |
| 分类号:R917.101 |
| 基金项目: |
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| Simultaneous Determination of Irisflorentin and Piperine in Danlyu Bushen Capsules by HPLC |
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WANG Wenpeng1, LI Xiangming1, ZHOU Yongyan1, FENG Yukang1, HAO Pengbin1, GAO Liang1, SUN Shengbin1, JIANG Guozhi2
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1.Shineway Pharmaceutical Group Co., Ltd., Shijiazhuang 051430, China;2.State-local Joint Engineering Laboratory of New Drugs Development Technology for TCM Injection, Shijiazhuang 051430, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the simultaneous determination of irisflorentin and piperine in Danlyu Bushen capsules. METHODS The HPLC was carried on Agilent ZORBAX Eclipse Plus C18(4.6 mm×250 mm, 5 μm), the mobile phase acetonitrile-0.2% phosphoric acid (41:59), the flow rate was 1.0 mL·min-1, the column temperature was 40℃, detection wavelength was 266 nm. RESULTS Under the chromatographic conditions, irisflorentin in 0.010 30-0.309 0 μg (r=0.999 8); piperine in 0.081 73-2.451 9 mg (r=0.999 8), had good linear relationship. Irisflorenti and piperine average sample recovery rate (n=9) were 99.7% and 100.6%; RSD were 1.2%, 0.9%, respectively. In the sample, the minimum content of irisflorentin and piperine was 0.35, 6.4 mg per granule, respectively. CONCLUSION The method is simple, accurate and has a good repeatability. It can be used as the quality control method of Danlyu Bushen capsules. |
| Key words: Danlyu Bushen capsules irisflorentin piperine HPLC content determination |
