| 引用本文: | 吕华静,李幸苗,顾露囡,蔡鑫君.雷公藤甲素自微乳对荷人前列腺癌裸鼠移植瘤的抑制作用研究[J].中国现代应用药学,2017,34(10):1385-1387. |
| LYU Huajing,LI Xingmiao,GU Lunan,CAI Xinjun.Inhibitory Effect of Triptolide Microemulsion on Human Prostate Cancer PC-3 Cell Line in Nude Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(10):1385-1387. |
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| 摘要: |
| 目的 研究雷公藤甲素自微乳对荷人前列腺癌PC-3细胞裸鼠移植瘤的抑制作用。方法 构建荷人前列腺癌PC-3细胞裸鼠移植瘤，分为模型组、雷公藤甲素组、雷公藤甲素自微乳组、空白辅料组，灌胃给药，每2天1次，连续18 d，每3天测定瘤体积；给药结束后处死裸鼠，比较各组裸鼠的体质量、肝脏与瘤块质量，计算抑瘤率，并对瘤块组织中血管内皮生长因子（vascular endothelial growth factor，VEGF）进行免疫组化研究。结果 与模型组相比，雷公藤甲素和雷公藤甲素自微乳均可抑制肿瘤体积的增长；与雷公藤甲素相比，雷公藤甲素自微乳可以提高抑瘤率，降低肿瘤组织中VEGF的表达水平，对肿瘤抑制作用更佳，且未发现明显的毒性反应；空白辅料组对肿瘤无抑制作用。结论 自微乳给药系统可以提高雷公藤甲素的抗肿瘤作用，安全性良好。 |
| 关键词: 雷公藤甲素 自微乳给药系统 PC-3细胞 抗肿瘤作用 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.10.005 |
| 分类号: |
| 基金项目:杭州市科技发展计划项目（20130733Q14）；浙江省中医药科技计划（2015ZA148） |
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| Inhibitory Effect of Triptolide Microemulsion on Human Prostate Cancer PC-3 Cell Line in Nude Mice |
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LYU Huajing1, LI Xingmiao1, GU Lunan2, CAI Xinjun2
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1.Department of Pharmacy, the First Hospital of Yongkang, Yongkang 321300, China;2.Department of Pharmacy, Integrated Chinese and Western Medicine Hospital of Zhejiang Province, Hangzhou 310003, China
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| Abstract: |
| OBJECTIVE To investigate the inhibitory effect of triptolide microemulsion on nude mice bearing human prostate cancer PC-3 cell. METHODS Nude mice xenografts beared human prostate cancer PC-3 cell were constructed, then these mice were divided into model group, triptolide group, triptolide microemulsion group and blank excipient group. The nude mice were administered orally, once every 2 days for 18 d, and their tumor volume was measured every 3 days. Then the nude mice were sacrificed at the end of administration; the body weight, liver and tumor weight of nude mice in each group were compared, the inhibition rate was calculated, further, the immunohistochemical study was carried out to detect the VEGF expression. RESULTS Compared with control group, triptolide and triptolide microemulsion group had obvious tumor growth inhibition effect; meanwhile, compared with triptolide group, triptolide microemulsion group could obviously down-regulat the expression level of VEGF in tumor tissue, and the effects of triptolide microemulsion on tumor inhibitory effect was better, furthermore, no obvious toxic reaction was found. The blank excipient group had no inhibitory effect on tumor. CONCLUSION Self microemulsion drug delivery system can enhance the anti-tumor effect of triptolide, and it is also relative safe. |
| Key words: triptolide self emulsifying drug delivery system PC-3 cells antitumor effect |
