| 引用本文: | 何迅,杨馨,张锦,廖尚高,李霞,李勇军.UPLC-Q-TOF/MS同时测定强力天麻杜仲胶囊中5种成分的含量[J].中国现代应用药学,2017,34(8):1140-1144. |
| HE Xun,YANG Xin,ZHANG Jin,LIAO Shanggao,LI Xia,LI Yongjun.Simultaneous Determination of Five Constituents in Qiangli Tianma Duzhong Capsules by UPLC-Q-TOF/MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(8):1140-1144. |
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| 摘要: |
| 目的 建立UPLC-Q-TOF/MS同时测定强力天麻杜仲胶囊中天麻素、京尼平苷酸、松脂醇二葡萄苷、苯甲酰新乌头原碱及蛇床子素含量的方法。方法 采用Waters ACQUITY UPLC，色谱柱为CORTECSTM C18柱（4.6 mm×150 mm，2.7 μm），流动相为乙腈（A）-0.1%甲酸水溶液（B）梯度洗脱，电喷雾离子源（ESI），正负离子模式，质量扫描范围为50~1 200 Da。结果 5种被测成分在线性范围内均具有良好的线性关系（r2 ≥ 0.997 4），平均回收率在99.533%~107.525%，RSD ≤ 2.56%。结论 应用UPLC-Q-TOF/MS法分离效果及重复性好，且快速、简便，可作为强力天麻杜仲胶囊的质量控制方法。 |
| 关键词: 强力天麻杜仲胶囊 UPLC-Q-TOF/MS 多指标含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.08.015 |
| 分类号:R917.101 |
| 基金项目:贵州省科学计划项目（黔科合平台人才[2016]5613，黔科合平台人才[2016]5677，黔科合重大专项字[2012]6009号）；贵州省优秀青年科技人才培养对象专项项目（黔科合人字[2015]11号） |
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| Simultaneous Determination of Five Constituents in Qiangli Tianma Duzhong Capsules by UPLC-Q-TOF/MS |
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HE Xun1, YANG Xin1, ZHANG Jin1, LIAO Shanggao1, LI Xia2, LI Yongjun1
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1.Guizhou Medical University, Guiyang 550004, China;2.Guizhou Hongyu Pharmaceutical Co., Ltd., Guiyang 550018, China
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| Abstract: |
| OBJECTIVE To establish an UPLC-Q-TOF/MS method for the simultaneous determination of the five major constituents (gastrodin, geniposidic acid, pinoresinol diglucoside, benzoylmesaconine and osthole) in Qiangli Tianma Duzhong capsules. METHODS The chromatographic separation was carried out on a Waters ACQUITY UPLC CORTECSTM C18 column(4.6 mm×150 mm, 2.7 μm) eluted in a gradient program. The mobile phase consisted of water containing acetonitrile (A) and 0.1% formic acid (B) with electrospray ionization source in both negative and positive modes. The mass scan range was 50-1 200 Da. RESULTS The five constituents showed good linear relationships within the ranges (r2 ≥ 0.997 4), whose average recoveries were in the range of 99.533%-107.525%, the RSD ≤ 2.56%. CONCLUSION This method shows good reproducibility and is very rapid and simple, which can be used for the quality control of Qiangli Tianma Duzhong capsules. |
| Key words: Qiangli Tianma Duzhong capsules UPLC-Q-TOF/MS multi-marker quantitative analysis |
