| 引用本文: | 王常顺,刘永利,冯丽.知柏地黄丸的UPLC指纹图谱研究[J].中国现代应用药学,2017,34(10):1432-1436. |
| WANG Changshun,LIU Yongli,FENG Li.Study on UPLC Fingerprint of Zhibai Dihuang Pills[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(10):1432-1436. |
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| 摘要: |
| 目的 建立知柏地黄丸UPLC指纹图谱的研究方法。方法 采用Waters Acquity BEH C18色谱柱（2.1 mm×100 mm，1.7 μm），流动相为乙腈-0.1%磷酸，梯度洗脱，检测波长236 nm，流速0.4 mL·min-1，柱温40℃，以丹皮酚作为参照物。结果 在12 min内完成指纹图谱分析，标出12个共有峰，对6个色谱峰进行了归属，并进行了相似度评价。结论 UPLC简便可行，建立的指纹图谱可用于知柏地黄丸的质量控制。 |
| 关键词: 知柏地黄丸 指纹图谱 UPLC 相似度 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.10.015 |
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| Study on UPLC Fingerprint of Zhibai Dihuang Pills |
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WANG Changshun, LIU Yongli, FENG Li
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Hebei Insititute for Drug Control, Shijiazhuang 050011, China
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| Abstract: |
| OBJECTIVE To develop a method for fingerprint study of Zhibai Dihuang pills by UPLC. METHODS The chromatographic condition was as follows:Waters Acquity BEH C18 column(2.1 mm×100 mm, 1.7 μm), using acetonitrile-0.1% H3PO4 as mobile phase, with a flow rate of 0.4 mL·min-1, the detective wavelength was set at 236 nm and the column temperature was 40℃. Paeonol was used as the reference substance. RESULTS The analysis time was 12 min. There were 12 common peaks in the fingerprints, 6 peaks were identified and the similarity of the fingerprint was compared. CONCLUSION The method is simple, feasible, and can be used for qulity control of Zhibai Dihuang pills. |
| Key words: Zhibai Dihuang pills fingerprint UPLC similarity |
