麸炒白术配方颗粒特征图谱及白术内酯Ⅲ含量测定研究

黄妍; 姚建标; 赖云枫; 王如伟<sup>*</sup>

引用本文:	黄妍,姚建标,赖云枫,王如伟.麸炒白术配方颗粒特征图谱及白术内酯Ⅲ含量测定研究[J].中国现代应用药学,2017,34(9):1243-1247.
	HUANG Yan,YAO Jianbiao,LAI Yunfeng,WANG Ruwei.Study on HPLC Specific Chromatogram of Bran-processed Atractylodes Formula Granule and Determination of Atractylenolide Ⅲ[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(9):1243-1247.

【打印本页】【HTML】【下载PDF全文】【查看/发表评论】【EndNote】【RefMan】【BibTex】

←前一篇|后一篇→

过刊浏览高级检索

本文已被：浏览 3365次下载 1929次	码上扫一扫！
分享到：微信更多字体:加大+\|默认\|缩小-
麸炒白术配方颗粒特征图谱及白术内酯Ⅲ含量测定研究
黄妍¹, 姚建标², 赖云枫², 王如伟²
1.浙江中医药大学药学院, 杭州 310053;2.浙江康恩贝制药股份有限公司, 杭州 310051

摘要:

目的建立麸炒白术饮片和麸炒白术配方颗粒中白术内酯Ⅲ的含量测定方法，并同时建立麸炒白术配方颗粒的HPLC特征图谱。方法采用色谱柱为Welch Xtimate C₁₈柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-水溶液，梯度洗脱；体积流量1.0 mL·min^-1；柱温30℃；检测波长224 nm；进样量10 μL。结果建立了麸炒白术饮片及颗粒中白术内酯Ⅲ的含量测定方法，白术内酯Ⅲ在0.031 3~0.782 4 μg内与色谱峰面积呈良好的线性关系；麸炒白术饮片和麸炒白术配方颗粒平均加样回收率分别102.0%（RSD=1.88%），101.0%（RSD=1.02%）；建立了麸炒白术配方颗粒的HPLC特征图谱，确立了8个特征峰。结论该方法准确、可靠，为麸炒白术配方颗粒的工艺研究及质量控制提供了参考依据。

关键词: 麸炒白术配方颗粒白术内酯Ⅲ 含量测定特征图谱质量研究

DOI：10.13748/j.cnki.issn1007-7693.2017.09.006

分类号:R284.1

基金项目:

Study on HPLC Specific Chromatogram of Bran-processed Atractylodes Formula Granule and Determination of Atractylenolide Ⅲ

HUANG Yan¹, YAO Jianbiao², LAI Yunfeng², WANG Ruwei²

1.Pharmacy College of Zhejiang Chinese Medicine University, Hangzhou 310053, China;2.Zhejiang CONBA Pharmaceutical Co., Ltd., Hangzhou 310051, China

Abstract:

OBJECTIVE To establish an HPLC method for the determination of atractylenolide Ⅲ in bran-processed atractylodes and bran-processed Atractylodes Formula granule, and to establish the specific chromatogram of bran-processed Atractylodes Formula granule. METHODS The chromatographic separation was performed on a Welch Xtimate C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase was a mixture of acetonitrile-water in gradient elution at a flow rate of 1.0 mL·min^-1. The detection wavelength was 224 nm and the column temperature was set at 30℃. RESULTS The linear range of atractylenolide Ⅲ in bran-processed atractylodes and bran-processed Atractylodes Formula granule was 0.031 3-0.782 4 μg, with average recovery of 102.0%(RSD=1.88%) and 101.0%(1.02%). The HPLC specific chromatogram of bran-processed Atractylodes Formula granule showed 8 characteristic peaks. CONCLUSION The method is reliable and accurate, and can provide a reference for the technical study and quality control of bran-processed Atractylodes Formula granule.

Key words: bran-processed Atractylodes Formula granule atractylenolide Ⅲ content determination specific chromatogram quality study