| 引用本文: | 叶鑫,孙文佳,王媛,李成功,宋燕华,李子刚,汤慧芳.S-腺苷蛋氨酸对子宫平滑肌收缩的影响[J].中国现代应用药学,2017,34(6):806-810. |
| YE Xin,SUN Wenjian,WANG Yuan,LI Chengong,SONG Yanhua,LI Zigang,TANG Huifang.Effect of S-Adenosylmethionine on the Contraction of Uterine Smooth Muscle of Mice[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(6):806-810. |
|
| 本文已被：浏览 2665次 下载 1999次 |
码上扫一扫！ |
|
|
| S-腺苷蛋氨酸对子宫平滑肌收缩的影响 |
|
叶鑫1, 孙文佳1, 王媛1, 李成功1, 宋燕华2, 李子刚3, 汤慧芳1
|
|
1.浙江大学医学院基础医学系, 杭州 310058;2.浙江省疾病控制中心, 杭州 310051;3.浙江大学医学院附属妇产科医院, 杭州 310006
|
|
| 摘要: |
| 目的 探究S-腺苷蛋氨酸对子宫平滑肌收缩的影响，为寻找安全有效的新型子宫收缩调节药物提供理论依据。方法 取未妊娠、妊娠早期（6.5 d）、妊娠中期（12.5~13.5 d）和妊娠晚期（16.5~17.5 d）ICR小鼠，分离子宫，制备成1 cm长子宫肌条，两端分别固定于张力换能器，采用氨甲酰胆碱诱导收缩，累积给药法给予沙丁胺醇、S-腺苷蛋氨酸、蛋氨酸、牛磺酸等药物，记录、测量并分析离体子宫收缩曲线。结果 沙丁胺醇和S-腺苷蛋氨酸对未孕、妊娠早期（6.5 d）、妊娠中期（12.5~13.5 d）和妊娠晚期（16.5~17.5 d）4个时间点的小鼠离体子宫平滑肌的收缩均有较强的抑制作用，分别在10-7 mol·L-1和1.0 mmol·L-1的溶液浓度下显著抑制收缩。蛋氨酸、牛磺酸（10 mmol·L-1）在实验浓度下对ICR小鼠离体子宫平滑肌的收缩影响不明显。结论 S-腺苷蛋氨酸可有效抑制不同阶段离体子宫的收缩，提示S-腺苷蛋氨酸可能是一个有效且安全的子宫收缩调节药物。 |
| 关键词: S-腺苷蛋氨酸 蛋氨酸 牛磺酸 沙丁胺醇 子宫平滑肌 收缩 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.06.004 |
| 分类号:R284.1；R917.101 |
| 基金项目:国家自然科学基金资助项目（81170536） |
|
| Effect of S-Adenosylmethionine on the Contraction of Uterine Smooth Muscle of Mice |
|
YE Xin1, SUN Wenjian1, WANG Yuan1, LI Chengong1, SONG Yanhua2, LI Zigang3, TANG Huifang1
|
|
1.School of Basic Medicine Sciences, Zhejiang University, Hangzhou 310058, China;2.Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China;3.Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China
|
| Abstract: |
| OBJECTIVE To investigate the effect of S-adenosylmethionine(SAMe) on contraction of uterine smooth muscle, and to provide a theoretical basis for novel drug for preterm labor with good characters in safety and effectiveness. METHODS The uterus was dissected from mice at the different time-points: non-pregnant, and earlypregnancy (6.5 d), mid pregnancy (12.5-13.5 d) and late pregnancy (16.5-17.5 d) and the uterus was divided into about 1 cm length in the direction of the longitudinal muscle fibers in the Lock's solution. The muscle strips were fixed to the transducers at both ends. Sulbactamol, SAMe, methionine and taurine were administered by the cumulative concentration administration method, the uterine contraction curves were recorded, measured and analyzed. RESULTS The strong inhibitory effects of salbutamol and SAMe on contraction of isolated uterine smooth muscle of the mice in the early stage (6.5 d), the second trimester (12.5-13.5 d) and the third trimester (16.5-17.5 d) were observed. Salbutamol 10-7 mol·L-1 and SAMe 1.0 mmol·L-1 signifantly inhibited contraction. The effects of the other amino acids (methionine and taurine) on the contraction of isolated uterine smooth muscle in mice were not significant. CONCLUSION SAMe can effectively inhibit the contraction of uterine phases of pregnancy, so SAMe may be a novel relaxation drug for uterine smooth muscle. |
| Key words: S-adenosylmethionine methionine taurine salbutamol uterine smooth muscle contraction |
