| 引用本文: | 舒祝明,蒋剑平,王建平,李全清,伍旭明.大孔树脂纯化胡柚皮总黄酮的工艺研究[J].中国现代应用药学,2017,34(5):649-653. |
| SHU Zhuming,JIANG Jianping,WANG Jianping,LI Quanqing,WU Xuming.Purification of Total Flavonoids from Peels of Citrus Changshan-huyou Y.B.Chang by Macroporous Resin[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(5):649-653. |
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| 摘要: |
| 目的 研究胡柚皮总黄酮的大孔树脂纯化工艺。方法 通过对8种不同型号大孔树脂进行静态吸附与解吸实验，优选合适的大孔树脂，并优化分离纯化条件。结果 HPD-300型大孔树脂对胡柚皮总黄酮有较好的吸附和洗脱效果，其最佳分离纯化条件如下：pH 4.0，3.83 mg·mL-1上样液，上样量为5 mL·g-1，上样体积流量为2.0 BV·h-1，依次用2 BV的水洗脱，4 BV 70%乙醇洗脱，洗脱剂的流速为2 BV·h-1。经HPD-300树脂处理后的总黄酮质量分数达76.21%，收率为93.94%。结论 HPD-300型大孔树脂用于富集胡柚皮总黄酮效果最佳，是一种理想的分离纯化介质。 |
| 关键词: 胡柚皮 总黄酮 大孔树脂 纯化工艺 吸附动力学特性 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.05.003 |
| 分类号: |
| 基金项目:国家自然科学基金（81573761）；浙江省自然科学基金（LY13H290011）；浙江省中医药科学研究基金（2015ZA074，2017ZB043）；杭州市医疗卫生科研项目（20150633B59） |
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| Purification of Total Flavonoids from Peels of Citrus Changshan-huyou Y.B.Chang by Macroporous Resin |
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SHU Zhuming1, JIANG Jianping2, WANG Jianping2, LI Quanqing3, WU Xuming2
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1.Shaoxing Women and Children's Hospital, Shaoxing 312000, China;2.Zhejiang Provincial Hospital of TCM, Hangzhou 310006, China;3.Zhejiang Provincial Xiaoshan Hospital, Hangzhou 311202, China
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| Abstract: |
| OBJECTIVE To investigate the purification technology of total flavonoids from the peels of Citrus Changshan-huyou Y.B. Chang by macroporous resin. METHODS Eight macroporous resins were chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters. RESULTS HPD-300 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were pH 4.0, sample mass concentration of 3.83 mg·mL-1, the loading amount of 5 mL·g-1, the loading flow rate of 2.0 BV·h-1. The sample was eluted by 2 BV of water and 4 BV of 70% ethanol respectively with the eluting flow rate of 2.0 BV·h-1. The content of total flavonoids increased to 76.21% after the purification, and the yield was 93.94%. CONCLUSION HPD-300 is an ideal resin with satisfactory enrichment for separating and purifying the total flavonoids from peels of Citrus Changshan-huyou Y.B. Chang. |
| Key words: peels of Citrus Changshan-huyou Y.B. Chang total flavonoids macroporous resin purification technology adsorption kinetics characteristics |
