| 引用本文: | 沈雁,钟继红,徐磊,李思,王章流,郑华君.盐酸小檗碱对溃疡性结肠炎小鼠结肠组织TNF-α、IL-1β和IL-10表达的影响[J].中国现代应用药学,2017,34(8):1094-1098. |
| SHEN Yan,ZHONG Jihong,XU Lei,LI Si,WANG Zhangliu,ZHENG Huajun.Effects of Berberine Hydrochloride on the Expression of TNF-α, IL-1β and IL-10 of Colon Tissue in Mice with Ulcerative Colitis[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(8):1094-1098. |
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| 摘要: |
| 目的 观察盐酸小檗碱（berberine hydrochloride，BBR）对溃疡性结肠炎模型小鼠结肠组织TNF-α、IL-1β和IL-10表达的影响，探讨其治疗UC的可能作用机制。方法 BALB/c小鼠随机分为模型对照组、BBR低、高剂量组、柳氮磺吡啶阳性对照组和空白对照组。采用右旋葡聚糖硫酸钠法复制UC小鼠模型后，每日灌胃给药1次，连续7 d。实验期间每天观察小鼠一般情况并评估疾病活动指数（DAI）；末次给药后解剖观察并评估结肠大体形态损伤指数（CMDI）；组织切片染色，光镜下观察结肠组织的病理学变化并评估组织损伤指数（TDI）；ELISA酶联法检测结肠组织中细胞因子TNF-α、IL-1β和IL-10的含量。结果 与模型对照组比较，BBR治疗组小鼠的结肠炎临床表现明显改善，其DAI、CMDI和TDI评分显著下降，小鼠结肠组织中TNF-α、IL-1β水平均明显降低，IL-10水平升高（P<0.05）。结论 BBR能有效治疗UC小鼠的结肠炎症，其机制可能与其抑制结肠组织中TNF-α和IL-1β、提高IL-10的表达有关。 |
| 关键词: 小檗碱 溃疡性结肠炎 细胞因子 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.08.005 |
| 分类号:R965.2 |
| 基金项目:浙江省中医药科技计划项目（2015ZB066）；浙江省医药卫生科技计划项目（2016KYB223） |
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| Effects of Berberine Hydrochloride on the Expression of TNF-α, IL-1β and IL-10 of Colon Tissue in Mice with Ulcerative Colitis |
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SHEN Yan1, ZHONG Jihong1, XU Lei1, LI Si2, WANG Zhangliu1, ZHENG Huajun1
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1.Department of Gastroenterology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310005, China;2.Deportment of Pediatrics, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China
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| Abstract: |
| OBJECTIVE To observe the effects of berberine hydrochloride (BBR) on the expression of TNF-α, IL-1β and IL-10 of colon tissue in mice with ulcerative colitis(UC), and to explore the possible mechanisms of its therapeutic effects on UC. METHODS BALB/c mice were randomly divided into five groups:model control group, low dose BBR group, high dose BBR group, salazosulfapyridine(SASP)/positive control group and blank control group. UC model was established by dextran sulphate sodium(DSS), and then daily gavage administration was given for 7 days. During the course of experiment, the general conditions of mice were observed, and disease activity index(DAI) were assessed. At the end of treatment, colon tissue were dissected, its macroscopic damage index(CMDI) and tissue damage index(TDI) were evaluated, TNF-α, IL-1β and IL-10 were determined by ELISA. RESULTS Compared with model control group, the clinical symptoms of colitis in BBR groups were greatly improved, the DAI, CMDI and TDI in BBR groups were all reduced obviously, and the contents of TNF-α, IL-1β were markedly decreased while IL-10 increased(P<0.05). CONCLUSION The therapeutic effect of BBR on colitis of UC mice is efficient, the mechanism may be related to its inhibition of TNF-α and IL-1β, and enhancement of IL-10 in colon tissue. |
| Key words: berberine hydrochloride ulcerative colitis cytokine |
