| 引用本文: | 蒋婷婷,卢端萍,黄旭慧.HPLC测定灵芝孢子油中灵芝酸C1的含量[J].中国现代应用药学,2017,34(5):726-728. |
| JIANG Tingting,LU Duanping,HUANG Xuhui.Determination of Ganoderic Acid C1 in Ganoderma Lucidum Spore Oil by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(5):726-728. |
|
| 摘要: |
| 目的 建立灵芝孢子油中灵芝酸C1的含量测定方法。方法 采用Agilent Zorbax-SB C8色谱柱（150 mm×4.6 mm，3.5 μm），以乙腈-0.1%醋酸（25︰75）为流动相，流速为1.0 mL·min-1，检测波长256 nm，柱温22℃。结果 灵芝酸C1在1.002~25.04 μg·mL-1内呈良好的线性关系（r=0.999 9），平均回收率为97.47%。不同来源的灵芝孢子油中灵芝酸C1含量有一定差别且含量均较低，部分样品中不含有灵芝酸C1。结论 该方法简便准确，重复性好，可为灵芝孢子油的质量控制提供参考。 |
| 关键词: 灵芝孢子油 高效液相色谱 灵芝酸C1 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.05.021 |
| 分类号:R284.1；R917.101 |
| 基金项目: |
|
| Determination of Ganoderic Acid C1 in Ganoderma Lucidum Spore Oil by HPLC |
|
JIANG Tingting1, LU Duanping2, HUANG Xuhui1
|
|
1.Fujian Provincial Hospital Jinshan Branch(Fujian Provincial Hospital South Branch), Fuzhou 350028, China;2.Fujian Institute for Food and Drug Quality Control, Fuzhou 350001, China
|
| Abstract: |
| OBJECTIVE To establish a method for the content determination of ganoderic acid C1 in Ganoderma lucidum spore oil. METHODS The determination was performed on an Agilent Zorbax-SB C8 column(150 mm×4.6 mm, 3.5 μm). The mobile phase was acetonitrile-0.1% acetic acid(25:75) as a flow rate of 1 mL·min-1. The detection wavelength was 256 nm and the column temperature was 22℃. RESULTS The ganoderic acid C1 showed a good linearity in the range of 1.002-25.04 μg·mL-1 (r=0.999 9) and the average recovery was 97.47%. The contents of ganoderic acid C1 in Ganoderma lucidum spore oil from different origins had a certain difference. The contents of ganoderic acid C1 in samples were low and some samples didn't contain ganoderma lucidum acid C1. CONCLUSION The established method is simple, accurate and repeatable, which can provide reference for the quality control of Ganoderma lucidum spore oil. |
| Key words: Ganoderma lucidum spore oil HPLC ganoderic acid C1 content determination |
