| 引用本文: | 王小平,王芳,白吉庆,王金,叶峥嵘,权利娜,沈舒文.HPLC同时测定藤珠胃康颗粒中4个皂苷类成分[J].中国现代应用药学,2017,34(4):553-556. |
| WANG Xiaoping,WANG Fang,BAI Jiqing,WANG Jin,YE Zhengrong,QUAN Lina,SHEN Shuwen.Simultaneous Determination of Four Saponins in Tengzhuweikang Granules by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(4):553-556. |
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| 摘要: |
| 目的 建立HPLC同时测定藤珠胃康颗粒中4个皂苷类成分（人参皂苷Re、人参皂苷Rb1、人参皂苷Ro和竹节参皂苷Ⅳa）的方法。方法 采用Hypersil GOLD C18色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈-0.2 %磷酸水溶液，梯度洗脱（-5~0 min，19%乙腈；0~14 min，19%→26%乙腈；14~22 min，26%→29%乙腈；22~30 min，29%乙腈；30~40 min，29%→35%乙腈；40~55 min，35%乙腈），体积流量1.0 mL·min-1，检测波长203 nm，柱温30 ℃。结果 人参皂苷Re在0.080 4~1.608 μg（r=1），人参皂苷Rb1在0.108 8~2.176 μg（r=0.999 9），人参皂苷Ro在0.288 8~5.776 μg（r=0.999 9），竹节参皂苷Ⅳa在0.176 0~3.520 μg（r=0.999 9）内线性关系良好；平均回收率（n=6）分别为99.41%，101.92%，99.76%，100.31%，RSD值分别为2.22%，2.07%，0.33%，0.64%。结论 本实验所建立的方法简单，专属性强，重复性好，可用于藤珠胃康颗粒中人参皂苷Re、人参皂苷Rb1、人参皂苷Ro和竹节参皂苷Ⅳa的测定。 |
| 关键词: 藤珠胃康颗粒 人参皂苷Re 人参皂苷Rb1 人参皂苷Ro 竹节参皂苷Ⅳa |
| DOI：10.13748/j.cnki.issn1007-7693.2017.04.017 |
| 分类号: |
| 基金项目:陕西省科技厅中药现代化项目（2012KTCL03-14）；陕西省科学技术研究发展计划项目[2009K19-03（2）] |
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| Simultaneous Determination of Four Saponins in Tengzhuweikang Granules by HPLC |
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WANG Xiaoping, WANG Fang, BAI Jiqing, WANG Jin, YE Zhengrong, QUAN Lina, SHEN Shuwen
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Shaanxi University of Chinese Medicine, Xianyang 712046, China
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| Abstract: |
| OBJECTIVE To establish a method for simultaneous determination of four saponins(ginsenoside Re, ginsenoside Rb1, ginsenoside Ro and chikusetsusaponin Ⅳa) in Tengzhuweikang granules by HPLC. METHODS The HPLC separation was achieved on a Hypersil GOLD C18(250 mm×4.6 mm, 5 μm) column. The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution with gradient elution(-5-0 min, 19% acetonitrile; 0-14 min, 19%→26% acetonitrile; 14-22 min, 26%→29% acetonitrile; 22-30 min, 29% acetonitrile; 30-40 min, 29%→35% acetonitrile; 40-55 min, 35% acetonitrile) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 203 nm and the colum temperature was 30 ℃. RESULTS The linear ranges of ginsenoside Re, ginsenoside Rb1, ginsenosideRo, chikusetsusaponin Ⅳa were 0.080 4-1.608 μg (r=1), 0.108 8-2.176 μg(r=0.999 9), 0.288 8-5.776 μg(r=0.999 9), 0.176 0-3.520 μg(r=0.999 9), respectively. The average recoveris(n=6) were 99.41%, 101.92%, 99.76%, 100.31%, respectively. RSD of the recoveris was 2.22%, 2.07%, 0.33%, 0.64%, respectively. CONCLUSION The method is simple and repeatable, can be used for the determination of ginsenoside Re, ginsenoside Rb1, ginsenoside Ro and chikusetsusaponin Ⅳa in Tengzhuweikang Granules. |
| Key words: Tengzhuweikang granules ginsenoside Re ginsenoside Rb1 ginsenoside Ro chikusetsusaponin Ⅳa |
