| 引用本文: | 何冬黎,张晓春,李倩.HPLC同时测定小蓟配方颗粒中蒙花苷和芹菜素的含量[J].中国现代应用药学,2017,34(3):410-412. |
| He Dongli,Zhang xiaochun,Li qian.Determination of Linarin and Apigenin in Cirsii Herba Dispensing Granule by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(3):410-412. |
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| 摘要: |
| 目的 建立HPLC同时测定小蓟配方颗粒中蒙花苷和芹菜素含量的方法。方法 采用Diamonsil-C18（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-1%冰醋酸水溶液为流动相进行梯度洗脱，流速：1.0 ml·min-1，检测波长：335 nm，柱温：30℃。结果 蒙花苷和芹菜素进样量分别在0.3~1.5 μg（r=0.999 4）和0.075~0.375 μg（r=0.999 7）内与峰面积呈良好的线性关系；加样回收率分别为99.19%和99.12%，RSD分别为1.27%和1.26%。结论 该方法操作简便，重复性好，可用于小蓟配方颗粒中蒙花苷和芹菜素的含量测定。 |
| 关键词: 小蓟配方颗粒 蒙花苷 芹菜素 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.03.024 |
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| Determination of Linarin and Apigenin in Cirsii Herba Dispensing Granule by HPLC |
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He Dongli, Zhang xiaochun, Li qian
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Department of Pharmacy, Suizhou Hospital, Hubei University of Medicine, Suizhou 441300, China
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| Abstract: |
| OBJECTIVE To develop a method for content determination of linarin and apigenin in Cirsii Herba Dispensing Granule by HPLC. METHODS The separation was carried out on Diamonsil-C18 column (4.6 mm×250 mm,5 μm) under gradient elution with acetonitrile-1% glacial acetic as mobile phase at a flow rate of 1.0 ml·min-1. The detection wavelength was 335 nm and the column temperature was maintained at 30℃. RESULTS There were good linear relationships between the masses and the peak areas of linarin and apigenin in the ranges of 0.3-1.5 μg (r=0.999 4) and 0.075-0.375 μg (r=0.999 7), respectively. The average recoveries were 99.19% and 99.12%, and RSDs were 1.27% and 1.26%. CONCLUSION The method is simple and reliable for the determination of linarin and apigenin in Cirsii Herba Dispensing Granule. |
| Key words: Cirsii Herba Dispensing Granule linarin apigenin HPLC |
