| 引用本文: | 杨杰锋,徐川龙,张洁,吴金韦.超临界流体色谱法测定碘帕醇的对映异构体[J].中国现代应用药学,2016,33(12):1566-1569. |
| YANG Jiefeng,XU chuanlong,ZHANG Jie,WU Jinwei.Determination of the Enantiomer of Iopmamidol by Supercritical Fluid Chromatography[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(12):1566-1569. |
|
| 摘要: |
| 目的 用超临界流体色谱法测定碘帕醇的对映异构体。方法 采用Chiralpak OD-H手性色谱柱，以CO2-甲醇（89：11）为流动相，检测波长为242 nm。结果 R-碘帕醇在0.2~1.2 mg·mL-1内线性关系良好，精密度RSD为1.2%，回收率为103.7%（RSD=0.7%），且R-碘帕醇与S-碘帕醇分离度良好。结论 本法快速、准确、可靠、专属性强，可用于碘帕醇中R-碘帕醇的控制。 |
| 关键词: 碘帕醇 Chiralpak OD-H手性柱 异构体 超临界流体色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2016.12.018 |
| 分类号: |
| 基金项目: |
|
| Determination of the Enantiomer of Iopmamidol by Supercritical Fluid Chromatography |
|
YANG Jiefeng1, XU chuanlong2, ZHANG Jie2, WU Jinwei2
|
|
1.Zhejiang Province Medicine Economic Development Center, Hangzhou 310012, China;2.Zhejiang Starry Pharmaceutical Co., Ltd., Taizhou 317300, China
|
| Abstract: |
| OBJECTIVE To determine the enantiomer of Iopmamidol by a supercritical fluid chromatography(SFC) method. METHODS A Chiralpak OD-H chiral column was used, with CO2-methanol mixture(89:11) as the mobile phase, at the detection wavelength of 242 nm. RESULTS The linear calibration for the R-iopamidol was in the range of 0.2-1.2 mg·mL-1, the precision was 1.2%, and recovery was 103.7%(RSD=0.7%). Good resolution of R-iopamidol and its S-iopamidol was achieved. CONCLUSION The SFC method has been developed for quality control, and detection of R-iopamidol of iopamidol with sensitivity and specificity. |
| Key words: iopamidol Chiralpak OD-H column isomer supercritical fluid chromatography (SFC) |
