| 引用本文: | 俞燕,刘强,杨吕洪.清心饮对柯萨奇B3病毒感染心脏微血管内皮细胞的保护作用[J].中国现代应用药学,2017,34(1):20-24. |
| YU Yan,LIU Qiang,YANG Lyuhong.Protective Effects of Qinxin Decoction on Cardiac Microvascular Endothelial Cells Infected by Coxsackievirus B3[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(1):20-24. |
|
| 摘要: |
| 目的 研究清心饮对柯萨奇B3病毒（Coxsackievirus B3，CVB3）感染心脏微血管内皮细胞（cardiac microvascular endothelial cells，CMVECs）的保护作用。方法 采用RT-PCR检测CVB3 RNA，应用倒置显微镜观察CMVECs形态，CCK8检测细胞活性，选择清心饮最佳干预时间点和稀释浓度。在最佳条件下通过免疫荧光法、Western blot法检测内皮细胞标志物CD31，间充质细胞标志物α-SMA的表达。结果 CVB3能在CMVECs中持续性复制，清心饮最佳干预时间点和浓度分别是72 h和20%含药血清。与空白组比较，CVB3组CMVECs形态固缩、变异，CD31表达减弱，α-SMA表达增强；与空白血清组比较，清心饮血清组CMVECs形态趋于正常，CD31表达增强，α-SMA表达减弱。结论 清心饮可抑制CVB3感染CMVECs发生内皮细胞转分化，对CMVECs具有保护作用。 |
| 关键词: 清心饮 柯萨奇B3病毒 心脏微血管内皮细胞 内皮细胞转分化 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.01.006 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目（LY13H270013） |
|
| Protective Effects of Qinxin Decoction on Cardiac Microvascular Endothelial Cells Infected by Coxsackievirus B3 |
|
YU Yan1,2, LIU Qiang1, YANG Lyuhong1
|
|
1.The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China;2.Tonglu Hospital of TCM, Tonglu 311500, China
|
| Abstract: |
| OBJECTIVE To research the protective effects of Qinxin decoction on CMVECs infected by Coxsackievirus B3 (CVB3). METHODS CVB3 RNA were detected by RT-PCR, cells morphology were observed by inverted microscope, cell viability were tested by CCK8 and the best intervention time and concentration of Qinxin decoction were chosen. Then endothelial cells marker-CD31 and mesenchymal cells marker-α-SMA were tested by immunofluorescence and Western blot in optimal conditions. RESULTS CVB3 could be replicated sustainably in CMVECs. The best intervention time and concentration of Qinxin decoction were 72 h and 20% diluted concentration. Compared with that in the blank group, CD31 protein showed a low expression, but α-SMA showed higher and the cells changed to pyknosis or variation in the group CVB3. Compared with that in the blank serum group, CD31 protein showed high expression, but α-SMA showed lower and the cells grew quite well in the Qinxin decoction serum group. CONCLUSION Qinxin decoction could inhibit the process of endothelial-to-mesenchymal transition and has a good protective effect on CMVECs following CVB3 infection. |
| Key words: Qinxin decoction Coxsackievirus B3 cardiac microvascular endothelial cells endothelial-to-mesenchymal transition |
