| 引用本文: | 任丽平,李先佳,金少举.基于caspase-3/bcl-2/bax信号通路的槐定碱诱导胰腺癌细胞株capan-1凋亡机制研究[J].中国现代应用药学,2017,34(3):325-328. |
| REN Lipinga,LI Xianjiab,JING Shaojua.Influence of Sophoridine on Proliferation and Caspase-3/bcl-2/bax Signaling Pathway of Human Pancreatic Cancer Cell Line Capan-1 Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(3):325-328. |
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| 摘要: |
| 目的 探讨槐定碱对人胰腺癌细胞株capan-1作用及机制。方法 MTT法检测细胞增殖；Hoechst33342染色法观察细胞凋亡；流式检测细胞凋亡和细胞周期百分率；Western blot法检测caspase-3/bcl-2/bax表达。结果 槐定碱可以成浓度依赖性诱导capan-1细胞增殖抑制和凋亡，增加S/G2期细胞比例，下调bcl-2和pro-caspase-3表达水平，上调bax蛋白的表达。结论 槐定碱能通过调节caspase-3/bcl-2/bax信号通路而诱导细胞凋亡。 |
| 关键词: 胰腺癌 capan-1 槐定碱 caspase-3/bcl-2/bax信号通路 细胞增殖 细胞凋亡 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.03.005 |
| 分类号: |
| 基金项目:2017年度河南省科技攻关项目（172102310214）；河南省高等学校重点科研资助项目（16B310004）；漯河医学高等专科学校2016年度校级研究资助计划项目（2016-S-LMC-13） |
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| Influence of Sophoridine on Proliferation and Caspase-3/bcl-2/bax Signaling Pathway of Human Pancreatic Cancer Cell Line Capan-1 Cells |
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REN Lipinga1, LI Xianjiab2, JING Shaojua1
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1.Luohe Medical College, Department of Pharmacology, Luohe 462002, China;2.Luohe Medical College, Department of Biochemistry, Luohe 462002, China
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| Abstract: |
| OBJECTIVE To explore the effects and mechanisms of sophoridine on human pancreatic cancer cell line capan-1. METHODS MTT, Hoechst33342 staining and flow cytometric analysis were used to evaluate the proliferation, apoptosis and cell cycle percentage. Western-blot assay were used to evaluate expression of caspase-3/bcl-2/bax protein. RESULTS Sophoridine could induce the proliferation inhibition, apoptosis and the expression of bcl-2 and pro-caspase-3 with increasing S/G2 percentage and the expression of bax on capan-1 cell in a concentration dependent manner. CONCLUSION Sophoridine can induce the apoptosis of capan-1 cell by regulating caspase-3/bcl-2/bax signaling pathway. |
| Key words: pancreatic cancer capan-1 sophoridine caspase-3/bcl-2/bax signaling pathway cell proliferation apoptosis |
