| 引用本文: | 孙叶丹,桑晶,张劲松,匡荣,李杨.2种光源对体外3T3细胞光毒性试验结果的比较[J].中国现代应用药学,2017,34(1):34-36. |
| SUN Yedan,SANG Jing,ZHANG Jingsong,KUANG Rong,LI Yang.Comparison of the Results of in Vitro 3T3 NRU Phototoxicity Test Between Different Light Sources[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(1):34-36. |
|
| 摘要: |
| 目的 比较2种光源对体外3T3细胞光毒性试验结果的影响。方法 参照化学品体外3T3中性红摄取光毒性试验指导原则（OECD 432），采用模拟阳光光源和紫外光源对6种参考物质进行试验。结果 6种参考物质在2种光源照射后其光刺激因子和平均光效应均接近OECD 432中的参考值，各个物质在无光和有光下的IC50值也相近。结论 2种光源对体外3T3细胞光毒性试验结果基本无影响。 |
| 关键词: 3T3细胞 光源 光刺激因子 平均光效应 IC50 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.01.009 |
| 分类号: |
| 基金项目: |
|
| Comparison of the Results of in Vitro 3T3 NRU Phototoxicity Test Between Different Light Sources |
|
SUN Yedan, SANG Jing, ZHANG Jingsong, KUANG Rong, LI Yang
|
|
Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China
|
| Abstract: |
| OBJECTIVE To compare the results of six kinds of reference materials under two different light sources irradiation in vitro 3T3 phototoxicity test. METHODS According to the guideline of OECD 432, carried out the in vitro 3T3 NRU phototoxicity test irradiated by simulated sunlight and ultraviolet light source separately. RESULTS The results of the photo irritation factor(PIF) and the mean photo effect(MPE) of six kinds of reference materials under different light sources were close to the reference value listed in the OECD 432, and the IC50 values for each substance were also similar. CONCLUSION After two light sources irradiation, there is no difference among six kinds of reference materials on in vitro 3T3 phototoxicity test results. |
| Key words: 3T3 light sources photo irritation factor(PIF) mean photo effect(MPE) IC50 |
