| 引用本文: | 王斌利,王新娣,石晓峰,张莉霞.HPLC同时测定紫斑牡丹花粉中槲皮素、木犀草素、异鼠李素的含量[J].中国现代应用药学,2017,34(2):262-265. |
| WANG Binli,WANG Xindi,SHI Xiaofeng,ZHANG Lixia.Simultaneous Determination of Quercetin,Luteolin and Isorhamnetin in Pollen of Paeonia Rockii by RP-HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(2):262-265. |
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| 摘要: |
| 目的 建立同时测定紫斑牡丹花粉中3个黄酮类化合物槲皮素、木犀草素、异鼠李素含量的RP-HPLC。方法 样品用25倍75%乙醇回流提取2 h，提取液加15%盐酸水解2.5 h；采用Agilent Eclipse plus C18色谱柱（250 mm×4.6 mm，5 μm），以甲醇-乙腈-0.1%磷酸（25：13：62）为流动相等度洗脱，流速：1.0 mL·min-1，检测波长：360 nm，柱温35℃。结果 槲皮素、木犀草素和异鼠李素分别在1.3~26.0，0.7~14.0，8.4~168.0μg·mL-1内呈良好的线性关系，相关系数均≥0.9999；加样回收率（n=9）的RSD值分别为99.8%，100.3%，100.8%。结论 该方法简单、高效，可作为紫斑牡丹花粉质量控制的参考 |
| 关键词: 紫斑牡丹花粉 槲皮素 木犀草素 异鼠李素 含量测定 高效液相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.02.023 |
| 分类号: |
| 基金项目:兰州市科技计划项目（2016-3-17） |
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| Simultaneous Determination of Quercetin,Luteolin and Isorhamnetin in Pollen of Paeonia Rockii by RP-HPLC |
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WANG Binli1, WANG Xindi2, SHI Xiaofeng1,2, ZHANG Lixia1
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1.Gansu University of TCM, Lanzhou 730030, China;2.Gansu Academy of Medical Science, Lanzhou 730050, China
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| Abstract: |
| OBJECTIVE To develope a RP-HPLC method for simultaneous determination of three flavonoids (quercetin, luteolin, isorhamnetin) in pollen from Paeonia rockii.METHODS The samples were pretreated, including reflux extraction with 75% ethanol for 2 h(with the ratio of liquor to material of 25:1), and then the extracting solution was hydrolyzed with 15% hydrochloric acid for another 2.5 h. Agilent Eclipse plus C18 column (250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was methanol-acetonitrile-0.1% phosphoric acid (25:13:62) at the flow rate of 1.0 mL·min-1. The detection wavelength was 360 nm and the column temperature was 35℃.RESULTS Calibration curves were found to be linear in the range of 1.3-26.0 μg·mL-1 for luteolin, 0.7-14.0 μg·mL-1 for quercetin and 8.4-168.0 μg·mL-1 for isorhamnetin. The correlation coefficients were greater or equal to 0.999 9. The average recoveries(n=9) of three flavonoids were 99.8%, 100.3% and 100.8%, respectively.CONCLUSION The developeded method is simple and efficient, which can be used for quality control of pollen of Paeonia rockii. |
| Key words: pollen of paeonia rockii quercetin luteolin isorhamnetin content assay HPLC |
