| 引用本文: | 张文婷,徐靖,程夏倩,李明焱,黄琴伟,郭增喜,赵维良.灵芝孢子粉质量标准提高研究[J].中国现代应用药学,2016,33(6):799-803. |
| ZHANG Wenting,XU Jing,CHENG Xiaqian,LI Mingyan,HUANG Qinwei,GUO Zengxi,ZHAO Weiliang.Studies on Improving Quality Standard of Ganoderma Lucidum Spore[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(6):799-803. |
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| 摘要: |
| 目的 建立灵芝孢子粉的HPLC特征图谱及三油酸甘油酯的含量测定方法。方法 采用ACCHROM Unitary-C18(250 mm×4.6 mm，5 μm)色谱柱，流动相为乙腈-异丙醇(51∶49)，流速为1.0 mL·min-1，柱温为30 ℃，检测器为蒸发光散射检测器。结果 建立了灵芝孢子粉HPLC特征图谱，确定了10个共有峰，指认了其中8个共有峰的成分分别为三亚油酸甘油酯、二亚油酸油酸甘油酯、二亚油酸棕榈酸甘油酯、二油酸亚油酸甘油酯、棕榈酸油酸亚油酸甘油酯、三油酸甘油酯、二油酸棕榈酸甘油酯和二油酸硬脂酸甘油酯。三油酸甘油酯进样量在0.613 2~18.40 μg(r=0.998 9)内与峰面积线性关系良好，平均加样回收率为104.1%(RSD=1.8%)。结论 特征图谱结合三油酸甘油酯的含量测定，能更好地控制灵芝孢子粉的质量。 |
| 关键词: 灵芝孢子粉 三油酸甘油酯 特征图谱 高效液相色谱法 |
| DOI： |
| 分类号:A |
| 基金项目: |
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| Studies on Improving Quality Standard of Ganoderma Lucidum Spore |
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ZHANG Wenting1, XU Jing2, CHENG Xiaqian3, LI Mingyan4, HUANG Qinwei1, GUO Zengxi1, ZHAO Weiliang1
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1.Zhenjiang Institute for Food and Drug Control, Hangzhou 310052, China;2.Zhejiang Province Rare Plant Medicine Engineering Technology Research Center, Wuyi 321200, China;3.The Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China;4.Wuyi Shouxiangu Traditional Chinese Medicine Decoction Pieces Co., Ltd., Wuyi 321200, China
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| Abstract: |
| OBJECTIVE To establish a specific HPLC chromatogram of Ganoderma lucidum spore and a method for determination of triolein. METHODS The chromatographic separation was performed on an ACCHROM Unitary-C18 column (250 mm×4.6 mm, 5 μm) with the mobile phase of acetonitrile-isopropanol(51∶49). The flow rate was 1.0 mL·min-1 at 30 ℃ with the detection by ELSD. RESULTS The results showed that the chromatographic fingerprint was completed with 10 recognizable peaks, of which 8 peaks kaempferol-3-O-β-D (trilinolein, 1,2-linolein-3-olein, 1,2-linolein-3-palmitin, 1,2-olein-3-linolein, 1-palmitin-2-olein-3-linolein, triolein, 1,2-olein-3-palmitin and 1,2-olein-3-stearin) were determined. The linear range of triolein was 0.613 2-18.40 μg(r=0.998 9), the average recovery was 104.1%(RSD=1.8%). CONCLUSION The combination of specific chromatograms and determination of triolein is suitable for use in the quality control of Ganoderma lucidum spore. |
| Key words: Ganoderma lucidum spore triolein fingerprint HPLC |
