| 引用本文: | 刘奇,夏正燕,周丹英,余琪.葡萄籽原花青素降血脂药效部位筛选[J].中国现代应用药学,2016,33(11):1378-1381. |
| LIU Qi,XIA Zhengyan,ZHOU Danying,YU Qi.Screening of Grape Seed Proanthocyanidins Lowering Efficacy Site[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(11):1378-1381. |
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| 摘要: |
| 目的 研究葡萄籽原花青素不同流分的降血脂药效。方法 采用不同浓度乙醇渗漉提取、大孔树脂分离纯化，制得10%，30%，50%，70%乙醇部位葡萄籽原花青素提取物，建立大鼠高血脂模型，分别测定各组血清甘油三酯（TG），总胆固醇（TC），谷丙转氨酶（ALT），谷草转氨酶（AST）的含量，并计算Lee'S指数和脏器指数，对各提取物进行药效学研究，确定葡萄籽原花青素降血脂有效部位。结果 葡萄籽原花青素提取物10%，30%，50%乙醇部位具有明显降低高血脂大鼠模型的TG含量的作用（P<0.01）；同时50%乙醇部位还可以明显降低高血脂大鼠模型的ALT和AST含量（P<0.01）；与模型组比较，给药后10%乙醇部位肾脏指数显著升高（P<0.01）；10%，30%，50%，70%乙醇部位组能显著升高脾脏指数（P<0.05）；30%乙醇部位能显著降低总脂指数（P<0.05）；10%，50%，70%乙醇部位能显著降低总脂指数（P<0.01）。结论 50%乙醇部位葡萄籽原花青素提取物为葡萄籽降血脂有效部位。 |
| 关键词: 葡萄籽原花青素 降血脂 药效部位筛选 |
| DOI：10.13748/j.cnki.issn1007-7693.2016.11.006 |
| 分类号: |
| 基金项目:浙江省科技厅公益技术研究社会发展项目(2014C33227) |
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| Screening of Grape Seed Proanthocyanidins Lowering Efficacy Site |
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LIU Qi, XIA Zhengyan, ZHOU Danying, YU Qi
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Zhejiang Institute of Traditional Chinese Medicine Co., Ltd., Hangzhou 310023, China
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| Abstract: |
| OBJECTIVE To study the lipid-lowering efficacy in different components of grape seed proanthocyanidins. METHODS Different concentrations of ethanol percolation extraction, macroporous resin separation and purification were used to obtain 10%, 30%, 50%, 70% ethanol portion of grape seed proanthocyanidin extract. Rats hyperlipidemia model was established, and the content of glycerol triester (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST) in each groups' serum were determined, Lee'S index and organ index were calculated as well. According to each extract pharmacodynamic study to determine the grape seed proanthocyanidins lipid-lowering effective parts. RESULTS Grape seed proanthocyanidin extract 10%, 30%, 50% ethanol parts could significantly reduce high cholesterol TG content in hyperlipidemia model rats(P<0.01); while 50% ethanol part could also significantly reduce the hyperlipidemia ALT and AST content in model rats (P<0.01); Compared with the model group, the resulting group elution kidney index increased significantly after administration of 10% ethanol (P<0.01); 10%, 30%, 50%, 70% ethanol eluted income group significantly increased spleen index (P<0.05); eluted with 30% ethanol resulting group can significantly reduce the total lipid index (P<0.05); 10%, 50%, 70% ethanol eluted income group can significantly reduce the total lipid index (P<0.01). CONCLUSION The 50% ethanol part of grape seed proanthocyanidins play an effective affect in lipid-lowering efficacy of grape seed extract. |
| Key words: grape seed proanthocyanidins lipid-lowering active site selection |
