| 引用本文: | 刘娟,郭宇,胡孟洋,翟美芳,平洋,张灵芝,李雪欣,于莲.葛根素固体脂质纳米粒抗肝损伤作用研究[J].中国现代应用药学,2016,33(9):1102-1106. |
| LIU Juan,GUO Yu,HU Mengyang,ZHAI Meifang,PING Yang,ZHANG Lingzhi,LI Xuexin,YU Lian.Effects of Puerarin Solid Lipid Nanoparticles on Liver Injury[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(9):1102-1106. |
|
| 摘要: |
| 目的 制备葛根素固体脂质纳米粒(puerarin solid lipid nanoparticles，Pue-SLN)，对Pue-SLN进行体外释药考察，并探讨Pue-SLN对CCl4诱导的急性肝损伤大鼠的治疗作用。方法 采用乳化-超声分散法制备Pue-SLN。大鼠腹腔注射CCl4造成急性肝损伤模型。48只大鼠随机分成6组：正常组、模型组、甘草酸二胺阳性对照组(13.5 mg·kg-1)、Pue-SLN高浓度组(27 mg·kg-1)、中浓度组(13.5 mg·kg-1)、低浓度组(6.75 mg·kg-1)。分别测定大鼠血清中丙氨酸转移酶(ALT)、碱性磷酸酶(ALP)、天门冬氨酸转移酶(AST)的活力，肝组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)含量，计算肝脏指数，并对肝组织进行组织形态学检查。结果 Pue-SLN各剂量组均能抑制肝损伤大鼠血清中ALT、AST、ALP活性，降低肝匀浆中MDA含量，增强SOD、GSH-PX活性，改善肝组织的病理形态。结论 Pue-SLN对CCl4诱导的大鼠急性肝损伤具有治疗作用。 |
| 关键词: 葛根素 固体脂质纳米粒 四氯化碳 急性肝损伤 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(81274101) |
|
| Effects of Puerarin Solid Lipid Nanoparticles on Liver Injury |
|
LIU Juan1, GUO Yu1, HU Mengyang2, ZHAI Meifang1, PING Yang1, ZHANG Lingzhi1, LI Xuexin1, YU Lian1
|
|
1.College of Pharmacy, Jiamusi University, Jiamusi 154007, China;2.Clinical Medicine, Shandong University, Jinan 250000, China
|
| Abstract: |
| OBJECTIVE To research the therapeutic effect of puerarin solid lipid nanoparticles(Pue-SLN) on liver injury in rats, prepare Pue-SLN and investigate drug release of Pue-SLN. METHODS Pue-SLN was prepared by emulsification ultrasonic dispersion method. Acute liver injury was induced by intraperitoneal injection of carbon tetrachloride in rats. Rats were randomly divided into 6 groups: normal control group, model group, glycyrrhizinate acid diamine positive control group(13.5 mg·kg-1), Pue-SLN groups with high concentration(27 mg·kg-1), middle concentration(13.5 mg·kg-1) and low concentration (6.75 mg·kg-1). ALT, ALP, AST in serum and MDA, SOD, GSH-PX in liver tissue homogenate were detected. And then, liver index and obtained histomorphology examination of liver tissue were calculated. RESULTS Pue-SLN groups could significantly inhibit the activities of ALT, AST, ALP in serum, decrease the content of MDA in liver homogenate and increase the activities of SOD, GSH-PX in liver homogenate. Pue-SLN groups could improve the pathological of liver tissue via observing confirm the pathological section histomorphology of liver tissue. CONCLUSION Pue-SLN have therapeutic effects on acute liver injury induced by carbon tetrachloride in rats. |
| Key words: puerarin solid lipid nanoparticles carbon tetrachloride acute liver injury |
