| 引用本文: | 张文婷,黄伟,黄琴伟,赵维良.中国药典2015年版颠茄酊中东莨菪内酯含量测定及特征图谱分析[J].中国现代应用药学,2016,33(5):626-628. |
| ZHANG Wenting,HUANG Wei,HUANG Qinwei,ZHAO Weiliang.HPLC Specific Chromatogram of Belladonna Tincture and Determination of Scopoletin in Chinese Pharmacopoeia (2015 Edition)[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(5):626-628. |
|
| 摘要: |
| 目的 建立颠茄酊的HPLC特征图谱及东莨菪内酯的含量测定方法。方法 采用Welch XB-C18(250 mm×4.6 mm，5 μm)色谱柱，流动相为甲醇-0.05%磷酸，梯度洗脱，流速：1.0 mL·min-1；柱温：30 ℃；检测波长：344 nm。结果 建立了颠茄酊HPLC特征图谱，确定了6个共有峰，指认了其中3个共有峰的成分分别为山柰素-3-O-半乳糖-(6→1)鼠李糖-7-O-葡萄糖苷、东莨菪内酯和芦丁。东莨菪内酯进样量在11.18~670.5 ng(r=1.000 0)内线性关系良好，平均加样回收率为104.2%。结论 特征图谱结合东莨菪内酯的定量测定，能更好地控制颠茄酊的质量。 |
| 关键词: 颠茄酊 东莨菪内酯 特征图谱 高效液相色谱法 |
| DOI： |
| 分类号:A |
| 基金项目: |
|
| HPLC Specific Chromatogram of Belladonna Tincture and Determination of Scopoletin in Chinese Pharmacopoeia (2015 Edition) |
|
ZHANG Wenting1, HUANG Wei2, HUANG Qinwei1, ZHAO Weiliang1
|
|
1.Zhenjiang Institute for Food and Drug Control, Hangzhou 310052, China;2.Zhejiang Medical College, Hangzhou 310053, China
|
| Abstract: |
| OBJECTIVE To establish a specific HPLC chromatogram of belladonna tincture and a method for determination of scopoletin. METHODS A Welch XB-C18 column (250 mm×4.6 mm, 5 μm) was adopted, methanol-0.05% phosphoric acid was used as the mobile phase for gradient elution, the flow rate was 1.0 mL·min-1, the column temperature was 30 ℃, and the wavelength was set at 344 nm. RESULTS The chromatographic fingerprint was completed with 6 recognizable peaks, of which 3 peaks [kaempferol-3-O-β-D (6-O-α-L-rhamnogalactoside)-7-O-β-D-glucoside, scopoletin and rutin] were determined. The linear range of scopoletin was 11.18-670.5 ng (r=1.000 0), the average recovery was 104.2%. CONCLUSION The combination of specific chromatograms and determination of scopoletin is suitable for the quality control of belladonna tincture. |
| Key words: belladonna tincture scopoletin fingerprint HPLC |
