| 引用本文: | 邓梦娇,俞静静,王昕妍,吕圭源,陈素红.HPLC同时测定银菊浸膏中绿原酸、木犀草苷、3, 5-O-二咖啡酰基奎宁酸含量[J].中国现代应用药学,2016,33(4):403-407. |
| DENG Mengjiao,YU Jingjing,WANG Xinyan,LYU Guiyuan,CHEN Suhong.Simultaneous Determination of Chlorogenic Acid, Mignonette Glycosides and 3, 5-O-dicaffeoylquinic Acid of Yinju Extract by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(4):403-407. |
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| 摘要: |
| 目的 建立同时测定银菊浸膏中绿原酸、木犀草苷、3, 5-O-二咖啡酰基奎宁酸含量的高效液相色谱法，优选最佳提取溶剂。方法 色谱条件：Ultimate PLUS C18色谱柱(4.6 mm×250 mm，5 μm)，以乙腈(A)-0.1%磷酸水溶液(B)为流动相梯度洗脱，流速1.0 mL·min-1，柱温40 ℃，波长348 nm，比较不同提取溶剂对浸膏中3种成分的影响。结果 绿原酸、木犀草苷、3, 5-O-二咖啡酰基奎宁酸分别在0.077~1.920，0.082~3.288，0.141~5.624 μg内线性关系良好，平均加样回收率为100.8%(RSD=2.3%)，101.5%(RSD=0.7%)，97.6%(RSD=0.6%)；银菊浸膏乙醇提取显著优于水提取，且40%乙醇提取最佳。结论 该法简便、准确，可用于银菊浸膏提取工艺优选与质量控制。 |
| 关键词: 菊花 金银花 绿原酸 木犀草苷 3, 5-O-二咖啡酰基奎宁酸 高效液相色谱法 |
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| 基金项目:“重大新药创制”国家科技重大专项(2011ZX09101-002-07)；国家自然科学基金资助项目(81274123)；浙江省卫生高层次创新人才培养工程项目(浙卫发[2010]190号)；浙江省重点实验室项目(2012E10002) |
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| Simultaneous Determination of Chlorogenic Acid, Mignonette Glycosides and 3, 5-O-dicaffeoylquinic Acid of Yinju Extract by HPLC |
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DENG Mengjiao1, YU Jingjing2, WANG Xinyan1, LYU Guiyuan2, CHEN Suhong3,1
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1.Wenzhou Medical University，Wenzhou 325035, China;2.Zhejiang Chinese Medical University, Hangzhou 310053, China;3.Zhejiang University of Technology, Hangzhou 310014, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the simultaneous determination the contents of chlorogenic acid, mignonette glycosides and 3, 5-O-dicaffeoylquinic acid in Yinju extract and select the best extraction solvent. METHODS Ultimate PLUS C18 column was applied(4.6 mm×250 mm, 5 μm) with acetonitrile(A) and 0.1% phosphoric acid(B) as mobile phase in gradient mode. The flow rate was 1.0 mL·min-1 with column temperature of 40?℃. The detection wavelength was set at 348 nm. The contents of 3 components in Yinju extract extracted by different extraction solvents were compared. RESULTS The linear range of chlorogenic acid, mignonette glycosides and 3, 5-O-dicaffeoylquinic acid were 0.077-1.920, 0.082-3.288, 0.141-5.624 μg and the average recoveries were 100.8%(RSD=2.3%), 101.5% (RSD=0.7%), 97.6%(RSD=0.6%). Ethanol was better than water to extract Yinju extract and 40% ethanol was the best extraction solvent. CONCLUSION This method is simple, accurate and capable for the optimization of extract process and quality control of Yinju extract. |
| Key words: Chrysanthemi Flos Lonicerae Japonicae Flos chlorogenic acid mignonette glycosides 3,5-O-dicaffeoylquinic acid HPLC |
