| 引用本文: | 倪兆成,梁慧,王楠楠,苏洁,吕圭源,陈素红.疏肝调脂方对高脂血症模型大鼠血脂、肝肾功能及微循环的影响[J].中国现代应用药学,2016,33(4):395-399. |
| NI Zhaocheng,LIANG Hui,WANG Nannan,SU Jie,LYU Guiyuan,CHEN Suhong.Effect of Shugan Tiaozhi Fang on Blood Lipid, Liver and Kidney Function and Microcirculation in Hyperlipidemia Model Rats[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(4):395-399. |
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| 摘要: |
| 目的 探讨疏肝调脂方对高脂血症模型大鼠血脂、肝肾功能及微循环的影响。方法 采用高糖高脂饲料复合酒饮建立高脂血症大鼠模型，造模2周后大鼠灌胃给予疏肝调脂方(0.66，1.32，2.64 g·kg-1)，每天1次，连续6周。观察给药前后各组大鼠的一般体征；测定血清总胆固醇(total cholesterol，TC)、甘油三酯(triglyceride，TG)、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol，HDL-C)、肌酐(creatinine，CR)、尿素氮(blood urea nitrogen，BUN)含量和丙氨酸氨基转移酶(alanine aminotransferase，ALT)活性，计算低密度脂蛋白胆固醇(low density lipoprotein-cholesterol，LDL-C)含量及动脉粥样硬化指数(atherosclerosis index，AI)；采用激光多普勒技术测定大鼠尾部微循环血流量。结果 疏肝调脂方3个剂量均能改善高脂血症大鼠的一般体征，降低血清TC、TG、LDL-C含量及AI，升高尾部微循环血流量(P<0.05或<0.01)；1.32 g·kg-1能降低血清ALT活性(P<0.05)；1.32，2.64g·kg-1能降低血清CR含量(P<0.05)。结论 疏肝调脂方可改善高脂血症模型大鼠的血脂水平、肝肾功能及微循环血流量，提示其具有调节血脂、改善肝肾损伤及微循环障碍的作用。 |
| 关键词: 疏肝调脂方 高脂血症 血脂 微循环 |
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| 基金项目:“重大新药创制”国家科技重大专项(2011ZX09102-011-07)；浙江省卫生高层次创新人才培养工程项目(浙卫[2010]190号)；浙江省重点实验室项目(2012E10002) |
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| Effect of Shugan Tiaozhi Fang on Blood Lipid, Liver and Kidney Function and Microcirculation in Hyperlipidemia Model Rats |
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NI Zhaocheng,LIANG Hui,WANG Nannan,SU Jie,LYU Guiyuan,CHEN Suhong
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1.Wenzhou Medical University, Wenzhou 325035,China;2.Zhejiang Chinese Medical University, Hangzhou 310053, China;3.Zhejiang Technology University, Hangzhou 310014, China
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| Abstract: |
| OBJECTIVE To investigate the effects of Shugan Tiaozhi Fang on blood lipid levels, liver and kidney function and microcirculation in hyperlipidemia model rats. METHODS The hyperlipidemia rat model was established by high-sugar and fat diets and alcohol-drinking. Two weeks after modeling, rats were given Shugan Tiaozhi Fang(0.66, 1.32, 2.64 g·kg-1) once a day, each group were given continuous administration for 6 weeks. The general signs of the rats were observe before and after administration. The content of total cholesterol(TC), triglyceride(TG), high density lipoprotein-cholesterol(HDL-C), creatinine(CR), blood urea nitrogen(BUN) and the active of ALT in serum were determined. The content of low density lipoprotein-cholesterol(LDL-C) and atherosclerosis index(AI) were calculated. Microcirculation blood flow was detected by laser Doppler technology. RESULTS Shugan Tiaozhi Fang (0.66, 1.32, 2.64 g·kg-1) improved the general signs of hyperlipidemia rats, reduced the content of TC, TG, LDL-C and AI(P<0.05 or 0.01), and increased the tail microcirculation blood flow(P<0.01). Shugan Tiaozhi Fang 1.32 g·kg-1 dose reduced the active of ALT(P<0.05), and 1.32, 2.64g·kg-1 reduced the content of CR(P<0.05). CONCLUSION Shugan Tiaozhi Fang could ameliorate the serum lipid levels, liver and kidney function and microcirculation disorder of rats with hyperlipidemia, suggesting that it is capable of regulating blood lipids, ameliorate the liver and kidney damage and microcirculation microcirculation disorder. |
| Key words: Shugan Tiaozhi Fang hyperlipidemia blood lipid microcirculation |
