| 摘要: |
| 目的 确定板蓝根黄酮的最佳提取工艺，并初步评价其抗氧化活性。方法 在单因素实验基础上，利用响应面法优化板蓝根总黄酮的提取条件; 通过总黄酮对1，1-二苯基-2-苦基肼自由基的清除作用来研究其抗氧化活性。结果 板蓝根黄酮最佳提取工艺为料液比1∶24.1、提取时间29 min和乙醇浓度60%，此条件下黄酮得率为54.4 mg·g-1；板蓝根黄酮对1，1-二苯基-2-苦基肼自由基具有较好的清除能力。结论 该优化工艺实现了板蓝根中黄酮类化合物的高效提取。 |
| 关键词: 板蓝根 黄酮 响应面法 优化 抗氧化 |
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| Optimization of Extracting Flavonoids from Isatidis Radix by Response Surface Methodology and Its Antioxidant Activity |
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ZHAO Wenting
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Linzi Maternal and Child Health Hospital of Zibo City, Zibo 255400, China
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| Abstract: |
| OBJECTIVE To explore the optimum extraction process of flavonoids from Isatidis Radix and evaluate its antioxidant activity. METHODS Based on the single-factor tests, the extraction conditions of total flavonoids from Isatidis Radix were optimized by response surface methodology. Meanwhile, the antioxidant activity of flavonoids was assessed by scavenging assays of 1,1-diphenyl-2-picrylhydrazyl(DPPH). RESULTS The optimum extraction conditions were as follows: ethanol concentration was 60%, liquid-solid ratio was 1∶24.1, and extraction time was 29 min. Under the optimal conditions, the extraction rate of total flavonoids was 54.4 mg·g-1. DPPH radical scavenging capacity of flavonoids was good. CONCLUSION The target of extracting flavonoids compound efficiently from Isatidis Radix has been achieved by the optimized technology. |
| Key words: Isatidis Radix flavonoids response surface analysis optimization antioxidant |
